Amidase (AMD)
Enzymes:Nibinyabuzima bya macromolecular bihindura, enzymes nyinshi ni proteyine
Amidase:Koresha hydrolysis ya endogenous na alifatique na amide ya amide itandukanye yohereza amatsinda ya acyl mumazi hamwe na acide yubusa na amoniya.Hydroxamic acide hamwe nandi acide kama ikoreshwa cyane nkibiyobyabwenge kuko aribintu bigize imikurire, antibiotike na inhibitori yibibyimba.Amidase irashobora kugabanywa mubwoko bwa R na S ubwoko bwa acylase ukurikije catalizike stereoselectivite.
Usibye guhagarika hydrolysis ya amide, amidase irashobora kandi guhagarika reaction yo kwimura acyl imbere ya co-substrate nka hydroxylamine.
Hagati yinkomoko zitandukanye zifite umwihariko wa substrate yihariye, zimwe murizo zishobora gusa hydrolyze aromatic amide, zimwe murizo zishobora gusa hydrolyze alifatique amide, hamwe na hydrolyze α-cyangwa ω-amino amide.Amine hafi ya yose afite ibikorwa byiza bya catalitiki gusa kuri acyclic cyangwa yoroshye ya aromatic amide, ariko kuri aromatics igoye, amide ya heterocyclic, cyane cyane amide hamwe ninsimburangingo ya ortho, muri rusange iba mike mubikorwa (enzymes nkeya zerekana ingaruka nziza za catalitiki).
Uburyo bwa Catalitike:
| Enzymes | Kode y'ibicuruzwa | Kode y'ibicuruzwa |
| Ifu ya Enzyme | ES-AMD-101 ~ ES-AMD-119 | urutonde rwa 19 amidase, 50 mg buri kintu 19 * 50mg / ikintu, cyangwa ubundi bwinshi |
| Igikoresho cyo kwerekana (SynKit) | ES-AMD-1900 | urutonde rwa 19 amidase, 1 mg buri kintu 19 * 1mg / ikintu |
Sub Substrate yihariye.
Guhitamo chiral ikomeye.
Impinduka nziza cyane.
Ibicuruzwa bike.
Conditions Imiterere yoroheje.
Yangiza ibidukikije.
Screen Kugenzura Enzyme bigomba gukorwa kubutaka bwihariye kubera umwihariko wa substrate, no kubona enzyme itera intego yibikorwa hamwe ningaruka nziza ya catalitiki.
➢ Ntuzigere uhura nibihe bikabije nka: ubushyuhe bwo hejuru, hejuru / hasi ya pH hamwe na organic solvent hamwe nibitekerezo byinshi.
➢ Mubisanzwe, sisitemu yogukora igomba gushiramo substrate, igisubizo cya buffer (Igisubizo cyiza pH ya enzyme).Co-substrates nka hydroxylamine igomba kuba ihari muri sisitemu yo kwimura acyl.
➢ AMD igomba kongerwaho nyuma muri sisitemu yogukora hamwe nibisubizo byiza pH n'ubushyuhe.
Ubwoko bwose bwa AMD bufite uburyo bwiza bwo kwitwara neza, buri kimwe rero kigomba gukomeza kwigwa kugiti cye.
Urugero 1(1):
Igikorwa cya Hydrolysis kuri Amide Substrates zitandukanye
| Substrate | Igikorwa cyihariye min. min-1mg-1 | Substrate | Igikorwa cyihariye min. min-1mg-1 |
| Acetamide | 3.8 | ο-OH benzamide | 1.4 |
| Propionamide | 3.9 | p-OH benzamide | 1.2 |
| Lactamide | 12.8 | ο-NH2benzamide | 1.0 |
| Butyramide | 11.9 | p-NH2benzamide | 0.8 |
| Isobutyramide | 26.2 | ο-Toluamide | 0.3 |
| Pentanamide | 22.0 | p-Toluamide | 8.1 |
| Hexanamide | 6.4 | Nikotinamide | 1.7 |
| Cyclohexanamide | 19.5 | Isonicotinamide | 1.8 |
| Acrylamide | 10.2 | Picolinamide | 2.1 |
| Metacrylamide | 3.5 | 3-Fenilpropionamide | 7.6 |
| Prolinamide | 3.4 | Indol-3-acetamide | 1.9 |
| Benzamide | 6.8 |
Igisubizo cyakozwe muri 50mM sodium fosifate buffer igisubizo, pH 7.5, kuri 70 ℃.
| Amide | Hydroxylamine | Hydrazine |
| Acetamide | 8.4 | 1.4 |
| Propionamide | 18.4 | 3.0 |
| Isobutyramide | 25.0 | 22.7 |
| Benzamide | 9.2 | 6.1 |
Igisubizo cyakozwe muri 50mM sodium fosifate buffer igisubizo, pH 7.5, kuri 70 ℃.
Ibyerekeranye na reagent yibanze: amide, mM 100 (benzamide, 10 mM);hydroxylamine na hydrazine, 400 mM;enzyme 0.9 μM.
Urugero 2(2):
Urugero 3(3):
1. D'Abusco AS, Ammendola S., n'abandi.Extremophile, 2001, 5: 183-192.
2. Guo FM, Wu JP, Yang LR, n'abandi.Gutunganya Ibinyabuzima, 2015, 50 (8): 1400-1404.
3. Zheng RC, Jin JQ, Wu ZM, n'abandi.Chimie ya Bioorganic, 2017, Iraboneka kumurongo 7.
