Reditase ya Nitrile (NRED)
Ibisobanuro bigufi:
Kubijyanye na reditase ya Nitrile
Hariho ubwoko 3 bwibicuruzwa bya NRED enzyme (Umubare nka ES-NRED101 ~ ES-NRED103) byakozwe na SyncoZymes.NRED irashobora kugabanya kugabanuka kwa groupe nitrile kuri amine.Mubikorwa bya catalizike, NADPH irakenewe nkabatwara hydrogen.
Ubwoko bwa Nitrile reductasecatalytic reaction
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E-Mail:lchen@syncozymes.com
| Enzymes | Kode y'ibicuruzwa | Ibisobanuro |
| Ibisobanuro | ES- NRED -101 ~ ES- NRED -103 | urutonde rwa 3 Nitrile Kugabanya, 50 mg buri kintu 3 * 50mg / ikintu, cyangwa ubundi bwinshi |
Sisitemu ikurikira irashobora gukoreshwa mubisobanuro:
1-10 mg / ml Substrate
10 mg / ml Enzyme
10% (v / v) Organic cosolvent (1)
100 mM ya buffer ya fosifate (pH7.0)
0.2 mg / ml NADP +
10 mg / ml Glucose
2 mg / ml Glucose dehydrogenase (GDH)
Ongeramo reaction kumashanyarazi ahoraho (urugero, 30oC, 150rpm) mumasaha 24 ~ 48.Centrifuge buri mvange kugirango igabanye poroteyine, izo ndengakamere zirashobora gukoreshwa muri TLC 、 HPLC cyangwa GC.
Icyitonderwa (1): Ibishishwa kama (nka DMSO, methanol, acetonitrile, IPA, nibindi) birahinduka, bikoreshwa mubutaka budashonga.
