Nitrile reductase (NRED)
Ibisobanuro bigufi:
Ku bijyanye no kugabanya imisemburo ya Nitrile
Hari ubwoko butatu bw'umusaruro wa enzyme ya NRED (Umubare nka ES-NRED101 ~ ES-NRED103) wakozwe na SyncoZymes. NRED ishobora gukurura kugabanuka kwa nitrile group kuri amine. Mu gihe cyo gukatalisa, NADPH irakenewe nk'umuyoboro wa hydrogen.
Ubwoko bw'ingaruka za Nitrile reductasecatalytic
Telefoni igendanwa/Wechat/WhatsApp: +86-13681683526
Imeri:lchen@syncozymes.com
| Imisemburo | Kode y'igicuruzwa | Ibisobanuro |
| Ibisobanuro | ES- NRED -101~ ES- NRED -103 | seti ya 3 Nitrile Reductase, 50 mg buri kimwe ibintu 3 * 50mg / ikintu, cyangwa ikindi gipimo |
Uburyo bukurikira bwo gusubiza bushobora gukoreshwa mu kwifashisha:
Igice cya 1-10 mg/ml
Enzyme ya 10 mg/ml
10% (v/v) Ikinyabutabire cy’umwimerere (1)
Fosfeti ya 100 mM (pH7.0)
0.2 mg/ml NADP+
10 mg/ml ya glucose
2 mg/ml Glucose dehydrogenase (GDH)
Shyira ingaruka ku gikoresho gipima ubushyuhe (urugero, 30oC, 150rpm) mu masaha 24 kugeza kuri 48. Shyira kuri buri mvange kugira ngo poroteyine irusheho kuba nziza, aho supernatant ishobora gukoreshwa mu gusesengura TLC, HPLC cyangwa GC.
Icyitonderwa (1): Umusemburo w’ibinyabuzima (nka DMSO, methanol, acetonitrile, IPA, nibindi) ni uwo guhitamo, ukoreshwa mu bintu bidashonga.
