Leucine Dehydrogenase (LeuDH)
Ibisobanuro bigufi:
Ibyerekeye Leucine dehydrogenase
Hariho ubwoko 1 bwibicuruzwa bya LeuDH enzyme (Umubare nka ES-LeuDH-101) byakozwe na SyncoZymes.LeuDH irashobora guhagarika ammoniation ya aside ya methylbutanoic 4 (umunyu) kuri L-leucine cyangwa okiside ya leucine kuri acide 4-methylbutanoic.ES-LeuDH irashobora kandi guhagarika aminide igabanya aside α-keto ifite imiterere isa nogutegura ibikomoka kuri aside amine.Mubikorwa bya catalizike, NADH irakenewe nkabatwara hydrogen.
Ubwoko bwa reaction ya Leucine dehydrogenasecatalytic
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E-Mail:lchen@syncozymes.com
| Enzymes | Kode y'ibicuruzwa | Ibisobanuro |
| Ifu ya Enzyme | ES-LeuDH-101 | urutonde rwa Oxidase ya Monoamine 1, 50 mg buri kintu 1 * 50mg / ikintu, cyangwa ubundi bwinshi |
Sisitemu ikurikira irashobora gukoreshwa mubisobanuro:
1-10 mg / ml: Substrate
10 mg / ml: Enzyme
10% (v / v): Cosolvent organique (1)
100 mM: Buffer ya fosifate (pH7.0)
0.2 mg / ml: NAD +
100 mM: Amonium ikora
10 mg / ml: Kora dehydrogenase (FDH)
Ongeramo reaction kumashanyarazi ahoraho (urugero, 30oC, 150rpm) mumasaha 24 ~ 48.Centrifuge buri mvange kugirango igabanye poroteyine, izo ndengakamere zirashobora gukoreshwa kubisesengura rya TLC, HPLC cyangwa GC.
Icyitonderwa (1): Ibishishwa kama (nka DMSO, methanol, acetonitrile, IPA, nibindi) birahinduka, bikoreshwa mubutaka budashonga.
