Nucloside fosifora (NP)

Ibisobanuro bigufi:

Ibyerekeye nucleoside fosifora

Hariho ubwoko 3 bwibicuruzwa bya NP enzyme (Umubare nka ES-NP-101 ~ ES-NP-103) byakozwe na SyncoZymes.ES-NP-101 ni purine nucleoside fosifora, ES-NP-102 na ES-NP-103 ni pyrimidine nucleoside fosifori.Nucleosidephosphorylase irashobora kubora nucleoside mubice na fosifate ya pentose.Nucleoside phosphorylase irashobora kugabanywamo purine nucleoside fosifora na pyrimidine nucleoside phosphorylase ukurikije ibyifuzo bya nucleoside.Posine nucleoside fosifora irashobora guhinduranya adenosine kuri adenine, inosine kuri hypoxanthine, guanosine kuri guanine, kandi ikabyara fosifate ya ribose icyarimwe.Pyrimidine nucleoside fosifora irashobora guhinduranya uridine kuri uracil kandi ikabyara fosifate ya ribose.

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Ibicuruzwa

Ubwoko bwa Nucloside fosifora:

Amakuru y'ibicuruzwa:

Enzymes	Kode y'ibicuruzwa	Ibisobanuro
Ifu ya Enzyme	ES-NP-101 ~ ES-NP-103	urutonde rwa 3 Nuclosidephosphoryalse, 50 mg buri kintu 3 * 50mg / ikintu, cyangwa ubundi bwinshi

Nucloside phosphoryalse yo gusuzuma reaction:

Sisitemu ikurikira irashobora gukoreshwa mubisobanuro:
1-10 mg / ml Substrate
10 mg / ml Enzyme
100 mM ya buffer ya fosifate (pH7.0)
Shyiramo reaction kumashanyarazi ahoraho (urugero, 30oC, 150rpm) mumasaha 12 ~ 24.Centrifuge buri mvange kugirango igabanye poroteyine, izo ndengakamere zirashobora gukoreshwa kubisesengura rya TLC, HPLC cyangwa GC.