Ketoreductase (KRED)
KRED kuva SyncoZymes: Hariho ubwoko 191 bwibicuruzwa bya enzyme ya KRED (Umubare nka ES-KRED-101 ~ ES-KRED-291) byakozwe na SyncoZymes.SZ-KRED irashobora gukoreshwa cyane mukugabanya regio- na stereoselective igabanya aldehydes, beta-ketone esters, α-ketone esters na ketone.
Ubwoko bwa catalitiki yerekana:
| Enzymes | Kode y'ibicuruzwa | Ibisobanuro |
| Ifu ya Enzyme | Ifu ya Enzyme | urutonde rwa 187 Ketoreductase, 50 mg buri kintu 187 * 50mg / ikintu, cyangwa ubundi bwinshi |
| Igikoresho cyo kwerekana (SynKit) | ES-KRED-18100 | urutonde rwa 181 Ketoreductase, 1 mg buri kintu 181 * 1mg / ikintu |
Sub Substrate yihariye.
Guhitamo chiral ikomeye.
Impinduka nziza cyane.
Ibicuruzwa bike.
Conditions Imiterere yoroheje.
Yangiza ibidukikije.
Screen Kugenzura Enzyme bigomba gukorwa kubutaka bwihariye kubera umwihariko wa substrate, no kubona enzyme itera intego yibikorwa hamwe ningaruka nziza ya catalitiki.
➢ Ntuzigere uhura nibihe bikabije nka: ubushyuhe bwo hejuru, hejuru / hasi ya pH hamwe na organic solvent hamwe nibitekerezo byinshi.
➢ Mubisanzwe, sisitemu yogukora igomba kuba irimo substrate, igisubizo cya buffer, coenzymes (NAD (H) cyangwa NADP (H)), sisitemu yo kuvugurura coenzyme (urugero glucose na glucose dehydrogenase).
➢ KRED igomba kongerwaho nyuma muri sisitemu yo kwitwara, nyuma ya pH nubushyuhe byahinduwe kumiterere.
Types Ubwoko bwose bwa KRED bufite uburyo bwiza bwo kwitwara neza, buri kimwe rero kigomba gukomeza kwigwa kugiti cye.
Urugero 1(1):
Urugero 2(2):
1. Li Z, Liu WD, Chen X, e tal.Tetrahedron, 2013, 69, 3561-3564.
2. Ni Y, Li CX, Ma HM, e tal.Koresha Microbiol Biotechnol, 2011, 89: 1111-1118.
