Cytochrome P450 monooxygenase (CYP)

Ibisobanuro bigufi:

Ku bijyanye na CYP

ES-CYPs: Ubwoko bwa enzymes za redox zifite mercaptan-heme nk'ikigo gikora mu gutuma ogisijeni ikwirakwizwa. Ni oxygenases za nyuma kandi zikenera heme kugira ngo ogisijeni ikoreshwe.

Cytochrome P450 monooxygenase itera ogisijeni, epoxidation na hydroxylation bya alkyl, hydroxylation na ogisijeni ya ammonia, ogisijeni ya sulfur, dealkylation (ogisijeni, sulfur, ammonia), ogisijeni yo gukuraho ogisijeni, deamination na dehalogenation.
Hari ubwoko 8 bw'ibicuruzwa bya monooxygenase bya cytochrome P450 (Umubare nka ES-CYP-101～ES-CYP-108) byakozwe na SyncoZymes.

Telefoni igendanwa/Wechat/WhatsApp: +86-13681683526

Imeri:lchen@syncozymes.com

Ohereza imeri kuri twe

Ibisobanuro birambuye ku gicuruzwa

Ibirango by'ibicuruzwa

Ku bijyanye na CYP:

Ubwoko bw'ingaruka za "catalytic reaction":

Amakuru ku bicuruzwa:

Imisemburo	Agakoresho ko gusuzuma (SynKit)	Ibisobanuro
Ifu ya enzyme	ES-CYP-101~ ES-CYP-108	seti ya 8 Cytochrome P450 Monooxygenases, 50 mg buri kimwe 8 ibintu * 50mg / ikintu, cyangwa ikindi gipimo
Agakoresho ko gusuzuma (SynKit)	ES-CYP-800	seti ya 8 Cytochrome P450 Monooxygenases, 1mg buri kimwe Ibintu 8 * 1mg / ikintu

Ibyiza:

★ Ubwinshi bw'urusobe rw'ibintu.
★ Guhinduranya ibintu byinshi.
★ Ibicuruzwa bike biva kuri ibyo.
★ Indwara zoroheje zo mu mutwe.
★ Irinda ibidukikije.

Amabwiriza yo gukoresha:

➢ Ubusanzwe, sisitemu y'ibisubizo igomba kuba irimo substrate, umuti wa buffer (pH nziza ya enzyme), coenzyme (NAD (H) cyangwa NADP (H)), sisitemu yo kuvugurura coenzyme (urugero: glucose na glucose dehydrogenase) na sisitemu yo kuvugurura coenzyme na coenzyme ya ES-CYP ishobora gusimburwa na hydrogen peroxide muri sisitemu y'ibisubizo igice.
➢ Ubwoko bwose bwa ES-CYPs bujyanye n'imiterere itandukanye myiza y'ibizamini bugomba kwigwa ukwabwo.
➢ Ubunini bwinshi bw'ibinyabutabire cyangwa ibikomoka ku bimera bishobora kubangamira imikorere ya ES-CYP. Ariko, ubu buryo bwo kubuza ibintu bushobora kugabanuka no kongeramo ibinyabutabire.

Ingero z'Ishyirwa mu Bikorwa:

Urugero rwa 1⁽¹⁾:

Urugero rwa 2⁽²⁾:

Urugero rwa 3⁽³⁾:

Urugero rwa 4⁽⁴⁾:

Ububiko:

Bika ahantu hatagera ku gipimo cya -20°C imyaka 2.

Icyitonderwa:

Ntukigere uhura n'ibintu bikomeye nko: ubushyuhe bwinshi, pH iri hejuru/hasi ndetse n'ibinyabutabire byinshi.

Amareferensi:

1. Zaretzki J, Matlock M, na Swamidass S JJ Chem. Inf. Model, 2013, 53, 3373–3383.
2. Gannett P M., Kabulski J, Perez F A., e tal. J. Am. Chem. Umuryango., 2006, 128 (26), 8374–8375.
3. Cryle M J., Matovic N J., na De Voss J J. Org. Lett., 2003, 5 (18), 3341–3344.
4. Kawauchi, H., Sasaki, J., Adachi, T., e tal. Biochim. Biophys. Acta, 1994, 1219, 179.
5. Yasutake, Y., Fujii, Y.; Cheon, WK e tal. Acta Crystallogr. 2009, 65, 372.