Cytochrome P450 monooxygenase (CYP)

Ibisobanuro bigufi:

Ibyerekeye CYP

ES-CYPs: Urwego rwimisemburo ya redox hamwe na mercaptan-heme nkikigo gikora kugirango uhindure ogisijeni.Ni okisijene ya terminal kandi ikenera heme kugirango ikore ogisijeni.

Cytochrome P450 monooxygenase itera okiside, epoxidation na hydroxylation ya alkyl, hydroxylation na okiside ya ammonia, okiside ya sulfure, dealkylation (ogisijeni, sulfure, amoniya), okiside dehydrogenation, deamination na dehalogenation.
Hariho ubwoko 8 bwibicuruzwa bya cytochrome P450 monooxygenase (Umubare nka ES-CYP-101 ～ ES-CYP-108) byakozwe na SyncoZymes.

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E-Mail:lchen@syncozymes.com

Ohereza imeri kuri twe

Ibicuruzwa birambuye

Ibicuruzwa

Ibyerekeye CYP:

Ubwoko bwa catalitiki yerekana:

Amakuru y'ibicuruzwa:

Enzymes	Igikoresho cyo kwerekana (SynKit)	Ibisobanuro
Ifu ya Enzyme	ES-CYP-101 ~ ES-CYP-108	urutonde rwa 8 Cytochrome P450 Monooxygenase, mg 50 buri kintu 8 * 50mg / ikintu, cyangwa ubundi bwinshi
Igikoresho cyo kwerekana (SynKit)	ES-CYP-800	urutonde rwa 8 Cytochrome P450 Monooxygenase, 1mg buri kintu 8 * 1mg / ikintu

Ibyiza:

★ Kugura ibintu byinshi.
Guhindura byinshi.
Ibicuruzwa bike.
Conditions Imiterere yoroheje.
Yangiza ibidukikije.

Amabwiriza yo gukoresha:

➢ Mubisanzwe, sisitemu yogukora igomba kuba irimo substrate, igisubizo cya buffer (Igisubizo cyiza pH ya enzyme), coenzyme (NAD (H) cyangwa NADP (H)), sisitemu yo kuvugurura coenzyme (urugero glucose na glucose dehydrogenase) na ES-CYP.sisitemu yo kuvugurura coenzyme na coenzyme irashobora gusimburwa na hydrogen peroxide muri sisitemu yogukora igice.
Ubwoko bwose bwa ES-CYPs bujyanye nuburyo bwiza bwo kwitwara bugomba kwigwa kugiti cye.
Kwibanda cyane Substrate cyangwa ibicuruzwa hamwe bishobora kubuza ibikorwa bya ES-CYP.Ariko, kubuzwa birashobora koroherezwa mugice cyo kongeramo substrate.

Ingero zo gusaba:

Urugero 1⁽¹⁾:

Urugero 2⁽²⁾:

Urugero 3⁽³⁾:

Urugero 4⁽⁴⁾:

Ububiko:

Komeza imyaka 2 munsi -20 ℃.

Icyitonderwa:

Ntuzigere uhura nibihe bikabije nka: ubushyuhe bwo hejuru, hejuru / hasi ya pH hamwe nubushakashatsi bwimbitse.

Reba:

1. Zaretzki J, Matlock M, na Swamidass S JJ Chem.Inf.Icyitegererezo, 2013, 53, 3373–3383.
2. Gannett P M., Kabulski J, Perez F A., e tal.J. Am.Chem.Umuryango., 2006, 128 (26), 8374–8375.
3. Cryle M J., Matovic N J., na De Voss J J. Org.Reka, 2003, 5 (18), 3341–3344.
4. Kawauchi, H., Sasaki, J., Adachi, T., e tal.Biochim.Biophys.Acta, 1994, 1219, 179.
5. Yasutake, Y., Fujii, Y.;Cheon, WK e tal.Acta Crystallogr.2009, 65, 372.