Epoxide hydrolase (EH)
Ibisobanuro bigufi:
Ibyerekeye hydrolase ya epoxide
EH ni ether hydrolase itera hydrolysis ya stereoselective hydrolysis ya epoxide yubwoko kugirango itange epoxide 1, 2-diol kandi ikora neza, ishobora gukoreshwa mugukemura chiral ya epoxide cyangwa gutegura diyoloji ya chiral.Zikoreshwa cyane mubuvuzi no mubindi bice.Hariho ubwoko 3 bwibicuruzwa bya epoxide hydrolase (Umubare nka ES-EH-101 ～ ES-EH-103) byakozwe na SyncoZymes.ES-EH nigikoresho cyingirakamaro muguhindura synthesis ya chiral alcool binyuze muri hydrolysis ya stereoselective hydrolysis ya aliphatic na aromatic epoxide.
Ubwoko bwa catalitiki yerekana:
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E-Mail:lchen@syncozymes.com
| Enzymes | Kode y'ibicuruzwa | Ibisobanuro |
| Ifu ya Enzyme | ES-EH-101 ~ ES-EH-103 | urutonde rwa Hydrolase 3 Epoxide, 50 mg buri kintu 3 * 50mg / ikintu, cyangwa ubundi bwinshi |
| Igikoresho cyo kwerekana (SynKit) | ES-EH-300 | urutonde rwa 3 Epoxide Hydrolase, mg 1 buri kintu 3 * 1mg / ikintu |
Sub Substrate yihariye.
Guhitamo chiral ikomeye.
Guhindura byinshi.
Ibicuruzwa bike.
Conditions Imiterere yoroheje.
Yangiza ibidukikije.
➢ Mubisanzwe, sisitemu yo kwitwara igomba kuba irimo substrate, igisubizo cya buffer (reaction nziza pH) na ES-EH.
Ubwoko bwose bwa ES-EHs bujyanye nuburyo bwiza bwo kwitwara bugomba kwigwa kugiti cye.
Kwibanda cyane Substrate cyangwa ibicuruzwa hamwe bishobora kubuza ibikorwa bya ES-EH.Ariko, kubuzwa birashobora koroherezwa mugice cyo kongeramo substrate.
Urugero 1(1):
Komeza imyaka 2 munsi -20 ℃.
Ntuzigere uhura nibihe bikabije nka: ubushyuhe bwo hejuru, hejuru / hasi ya pH hamwe nubushakashatsi bwimbitse.
1. Kim YH, Lee I, Choi SH, e tal.J Mol Catal B-Enzym, 2013, 89: 48-51.
