Lactate Dehydrogenase (LDH)
Ibisobanuro bigufi:
Ku bijyanye na Lactate dehydrogenase
Hari ubwoko bumwe bw'umusaruro wa enzyme ya LDH (Numero nka ES-LDH) yakozwe na SyncoZymes. LDH ishobora gutuma pyruvate igabanuka ikaba aside L-lactic, cyangwa ikagabanya izindi aside α-keto zisa nazo. Mu gihe cyo gukatalisa, NADH irakenewe nk'umuyoboro wa hydrogen.
Ubwoko bw'ingaruka za Lactate dehydrogenasecatalytic
Telefoni igendanwa/Wechat/WhatsApp: +86-13681683526
Imeri:lchen@syncozymes.com
| Imisemburo | Kode y'igicuruzwa | Ibisobanuro |
| Ifu ya enzyme | ES-LDH-101 | seti ya Monoamine Oxidase 1, 50 mg buri kimwe Ikintu 1 * 50mg / ikintu, cyangwa ikindi gipimo |
Uburyo bukurikira bwo gusubiza bushobora gukoreshwa mu kwifashisha:
1-10 mg/ml: Igice cy'ingenzi
10 mg/ml: Enzyme
10% (v/v): Cosolvent y'umwimerere (1)
100 mM: Fosfate buffer (pH7.0)
0.2 mg/ml: NAD+
100 mM: Ammonium formate
10 mg/ml: Formate dehydrogenase (FDH)
Shyira ingaruka ku gikoresho gipima ubushyuhe (urugero, 30oC, 150rpm) mu gihe cy'amasaha 24 kugeza kuri 48. Shyira kuri buri mvange kugira ngo poroteyine irusheho kuba nziza, aho supernatant ishobora gukoreshwa mu gusesengura TLC, HPLC cyangwa GC.
Icyitonderwa (1): Umusemburo w’ibinyabuzima (nka DMSO, methanol, acetonitrile, IPA, nibindi) ni uwo guhitamo, ukoreshwa mu bintu bidashonga.
