CALB yimuwe
CALB ihagarikwa na adsorption kumubiri kuri hydrophobique resin cyane ni macroporome, styrene / methacrylate polymer.Immobilized CALB irakwiriye gukoreshwa mumashanyarazi kama na sisitemu idafite umusemburo, kandi irashobora gukoreshwa kandi igakoreshwa inshuro nyinshi mubihe bikwiye.
Kode y'ibicuruzwa: SZ-CALB- IMMO100A, SZ-CALB- IMMO100B.
Activity Igikorwa cyo hejuru, guhitamo chiral ihanitse no guhagarara neza.
Performance Imikorere myiza mubice bitari amazi.
Kuraho byoroshye muri sisitemu yo kubyitwaramo, guhagarika vuba reaction, kandi wirinde ibisigazwa bya poroteyine mubicuruzwa.
★ Irashobora gukoreshwa kandi igakoreshwa kugirango igabanye igiciro.
| Igikorwa | ≥10000PLU / g |
| pH urwego rwo kubyitwaramo | 5-9 |
| Ubushyuhe bwo kurwego rwo kubyitwaramo | 10-60 ℃ |
| Kugaragara | CALB-IMMO100-A: Umuhondo woroshye kugeza umukara ukomeye CALB-IMMO100-B: Umweru kugeza umuhondo wijimye |
| Ingano ya Particle | 300-500 mm |
| Gutakaza kumisha kuri 105 ℃ | 0.5% -3.0% |
| Resin for immobilisation | Macroporous, styrene / methacrylate polymer |
| Igisubizo | Amazi, ibishishwa kama, nibindi, cyangwa bidafite ibishishwa.Kubisubizo mumashanyarazi amwe n'amwe, amazi 3% arashobora kongerwaho kugirango arusheho kugira ingaruka |
| Ingano ya Particle | CALB-IMMO100-A: 200-800 mm CALB-IMMO100-B: 400-1200 mm |
Igisobanuro cyibice: Igice 1 gihuye na synthesis ya 1μmol kumunota propyl laurate kuva acide lauric na 1-propanol kuri 60 ℃.CALB-IMMP100-A yavuzwe haruguru na CALB-IMMO100-B ihuye nabatwara ibinyabiziga bafite ubunini butandukanye.
1. Ubwoko bwa reaction
Enzyme yimisemburo irakoreshwa kuri reaction ya kettle batch reaction hamwe nigitanda gihamye gikomeza gutemba.Tugomba kumenya kwirinda guhonyora bitewe nimbaraga zo hanze mugihe cyo kugaburira cyangwa kuzuza.
2. Igisubizo pH, ubushyuhe hamwe na solve
Enzyme yimibilisiti igomba kongerwaho nyuma, nyuma yibindi bikoresho byongeweho bigashonga, na pH bigahinduka.
Niba ikoreshwa rya substrate cyangwa gushiraho ibicuruzwa bizaganisha ku guhinduka kwa pH mugihe cyo kubyitwaramo, buffer ihagije igomba kongerwaho sisitemu yo kubyitwaramo, cyangwa pH igomba gukurikiranwa no guhindurwa mugihe cya reaction.
Mubipimo byo kwihanganira ubushyuhe bwa CALB (munsi ya 60 ℃), igipimo cyo guhinduka cyiyongereye hamwe no kwiyongera kwubushyuhe.Mugukoresha mubikorwa, ubushyuhe bwa reaction bugomba gutoranywa ukurikije ihame rya substrate cyangwa ibicuruzwa.
Mubisanzwe, reaction ya ester hydrolysis irakwiriye muri sisitemu yicyiciro cyamazi, mugihe ester synthesis reaction ikwiranye na sisitemu yicyiciro.Umuti ukomoka ku bimera urashobora kuba Ethanol, tetrahydrofuran, n-hexane, n-heptane na toluene, cyangwa ibishishwa bivanze.Kubisubizo mumashanyarazi amwe n'amwe, amazi 3% arashobora kongerwaho kugirango arusheho kugira ingaruka.
3. Koresha kandi ubuzima bwa serivisi bwa CALB
Mugihe gikwiye cyo kwitwara neza, CALB irashobora kugarurwa no gukoreshwa, kandi ibihe byihariye byo gusaba biratandukanye nimishinga itandukanye.
Niba CALB yagaruwe idakoreshwa neza kandi ikeneye kubikwa nyuma yo gukira, igomba gukaraba no gukama no gufungwa kuri 2-8 ℃.
Nyuma yuburyo bwinshi bwo kongera gukoresha, niba imikorere ya reaction yagabanutseho gato, CALB irashobora kongerwaho muburyo bukwiye kandi igakomeza gukoreshwa.Niba imikorere ya reaction yagabanutse cyane, igomba gusimburwa.
Urugero rwa 1 (Aminolysis)(1):
Urugero rwa 2 (Aminolysis)(2):
Urugero rwa 3 (Impeta ifungura polyester synthesis)(3):
Urugero rwa 4 (Transesterification, regioselective ya hydroxyl)(4):
Urugero rwa 5 (Transesterification, gukemura ibibazo bya alcool)(5):
Urugero rwa 6 (Esterification, gukemura kinetic ya acide karubike)(6):
Urugero 7 (Esterolysis, gukemura kinetic)(7):
Urugero rwa 8 (Hydrolysis ya amide)(8):
Urugero rwa 9 (Acylation ya amine)(9):
Urugero rwa 10 (Aza-Michael wongeyeho reaction)(10):
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