CALB idakora neza

Ibisobanuro bigufi:

CALB

Lipase B ikomoka kuri Candida antarctica (CALB) ikorwa binyuze mu gushyushya amazi hifashishijwe Pichia pastoras yahinduwe mu buryo bwa genetike.

CALB ishobora gukoreshwa mu gice cy’amazi cyangwa igice cy’ibinyabuzima, esterolysis, transesterification, synthesis ya polyester ifunguye impeta, aminolysis, hydrolysis ya amides, acylation ya amines na addition reaction.

CALB ifite uburyo bwo guhitamo neza ibishushanyo mbonera bya chiral n'uburyo bwo guhitamo imyanya, bityo ikaba ishobora gukoreshwa cyane mu nganda zitunganya amavuta, ibiribwa, ubuvuzi, ubwiza n'izindi nganda zikora imiti.

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Ibisobanuro birambuye ku gicuruzwa

Ibirango by'ibicuruzwa

CALB idakora neza:

CALB irabuzwa kugenda no kwinjizwa mu ruhu rw’amazi rwinshi rufite imyenge minini, polimeri ifite imyenge minini, styrene/methacrylate. CALB idashobora kugenda ikwiriye gukoreshwa mu bintu bidafite imisemburo, kandi ishobora kongera gukoreshwa igihe kirekire mu bihe bikwiye.
Kode y'ibicuruzwa: SZ-CALB- IMMO100A, SZ-CALB- IMMO100B.

Ibyiza bya SZ-CALB-IMMO100:

★Ibikorwa byinshi, guhitamo neza kw'imyitozo ngororamubiri no gutuza cyane.
★Imikorere myiza mu bihe bitari amazi.
★Kuramo byoroshye mu buryo bwo gukora reaction, hagarika reaction vuba, kandi wirinde ibisigazwa bya poroteyine mu bicuruzwa.
★Ishobora kongera gukoreshwa no kongera gukoreshwa kugira ngo igabanye ikiguzi.

Ibipimo by'ibicuruzwa bya SZ-CALB-IMMO100:

Igikorwa	≥10000PLU/g
urwego rwa pH ku bijyanye n'ingaruka	5-9
Ingano y'ubushyuhe ku bijyanye n'ingaruka	10-60℃
Isura	CALB-IMMO100-A: Ibara ry'umuhondo woroshye kuva ku ibara ry'umukara CALB-IMMO100-B: Ibara ry'umweru kugeza ku ibara ry'umukara woroshye
Ingano y'uduce duto	300-500μm
Igihombo mu gihe cy'umuvuduko w'amazi ku bushyuhe bwa 105℃	0.5%-3.0%
Resin yo guhagarika kugenda	Polima ifite imyenge minini, styrene/methacrylate
Umuti ugabanya ingaruka z'ingaruka	Amazi, organic solvent, nibindi, cyangwa nta solvent. Ku bijyanye n'ingaruka muri organic solvents zimwe na zimwe, amazi 3% ashobora kongerwamo kugira ngo anonosore ingaruka z'ingaruka
Ingano y'uduce duto	CALB-IMMO100-A: 200-800 μm CALB-IMMO100-B: 400-1200 μm

Ibisobanuro by'igice: Igice 1 gihuye n'ikorwa rya 1μmol kuri buri munota rya propyl laurate iva muri aside laurike na 1-propanol kuri 60°C. CALB-IMMP100-A na CALB-IMMO100-B byavuzwe haruguru bihuye n'ibinyabiziga bidakora neza bifite ingano zitandukanye z'uduce.

Amabwiriza n'Ingamba zo Kwirinda:

1. Ubwoko bwa reactor
Iyi enzyme idakora neza ikoreshwa kuri kettle batch reactor ndetse na fixed bed continuous flow reactor. Igomba kwitabwaho kugira ngo wirinde gusya bitewe n'imbaraga zo hanze mu gihe cyo kugaburira cyangwa kuzuza.
2. pH y'ingaruka, ubushyuhe n'umusemburo
Enzyme idakora igomba kongerwamo nyuma, nyuma yo kongerwamo ibindi bikoresho no gushonga, hanyuma pH igahindurwa.
Niba gukoresha substrate cyangwa ikorwa ry'umusaruro bizatuma pH ihinduka mu gihe cyo gukora reaction, hagomba kongerwamo buffer ihagije muri sisitemu y'action, cyangwa pH igakurikiranwa kandi igahindurwa mu gihe cyo gukora reaction.
Mu rwego rwo kwihanganira ubushyuhe bwa CALB (munsi ya 60 ℃), igipimo cyo guhindura cyariyongereye uko ubushyuhe bwiyongera. Mu buryo bufatika, ubushyuhe bwo gusubiza bugomba gutoranywa hakurikijwe ubuziranenge bw'ikintu cyangwa umusaruro.
Muri rusange, reaction ya ester hydrolysis ikwiriye muri sisitemu y'amazi, mu gihe reaction ya ester synthesis ikwiriye muri sisitemu y'ibinyabuzima. Umusokoro w'ibinyabuzima ushobora kuba ethanol, tetrahydrofuran, n-hexane, n-heptane na toluene, cyangwa umusokoro uvanze ukwiye. Ku bijyanye n'irangizwa muri bimwe mu bisokoro by'ibinyabuzima, 3% by'amazi bishobora kongerwamo kugira ngo binoze ingaruka z'irangizwa.
3. Kongera gukoresha no kumara igihe CALB ikora
Mu gihe cy’uburyo bukwiye bwo gusubiza, CALB ishobora kugaruzwa no kongera gukoreshwa, kandi igihe cyo kuyikoresha gitandukana bitewe n’imishinga itandukanye.
Iyo CALB yasubiwemo idasubiwemo buri gihe kandi ikaba igomba kubikwa nyuma yo kuyigarura, igomba kozwa, ikamushwa hanyuma igafungwa kuri 2-8 ℃.
Nyuma yo kongera gukoreshwa inshuro nyinshi, iyo ubushobozi bwo gukora ikizamini cyagabanutseho gato, CALB ishobora kongerwamo neza igakomeza gukoreshwa. Iyo ubushobozi bwo gukora ikizamini cyagabanutse cyane, igomba gusimbuzwa.

Ingero z'Ishyirwa mu Bikorwa:

Urugero rwa 1 (Aminolysis)⁽¹⁾:

Urugero rwa 2 (Aminolysis)⁽²⁾:

Urugero rwa 3 (Gutunganya polyester ifungura impeta)⁽³⁾:

Urugero rwa 4 (Guhindura imiterere y'ibinyabutabire, guhitamo itsinda rya hydroxyl)⁽⁴⁾:

Urugero rwa 5 (Guhindura imiterere y'ibimera mu buryo bwa transsester, ubushobozi bwo gukemura ikibazo cy'imisemburo ya alkoholi zo mu bwoko bwa racemic)⁽⁵⁾:

Urugero rwa 6 (Esterification, resolution ya kinetic ya aside carboxylic)⁽⁶⁾:

Urugero rwa 7 (Esterolysis, igisubizo cya kinetic)⁽⁷⁾:

Urugero rwa 8 (Hydrolysis ya amides)⁽⁸⁾:

Urugero rwa 9 (Acylation ya amines)⁽⁹⁾:

Urugero rwa 10 (igisubizo cyo kongeramo Aza-Michael)⁽¹⁰⁾:

Amareferensi:

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