Transaminase (ATA)
Enzymes: Nibintu bya macromolecular biologiya, imisemburo myinshi ni proteyine.
Transaminase: Icyiciro cyimisemburo itera aminide yoherejwe hagati ya acide amine na acide ya keto.Transaminase ni enzyme yingenzi yibinyabuzima muri synthesis ya asimmetric hamwe no gukemura amoko ya chiral amine.
Aminotransferase irashobora kugabanywamo ibyiciro bine ukurikije uko bikurikirana n'imiterere: Ⅰ, Ⅱ, Ⅲ na Ⅳ.ω-aminotransferase ni iy'icyiciro cya II transaminase, ikunze gukoreshwa mugutegura amine ya chiral na acide amine idasanzwe, nka β-amino acide.
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Uburyo bwa Catalitike:
| Enzymes | Kode y'ibicuruzwa | Kode y'ibicuruzwa |
| Ifu ya Enzyme | ES-ATA-101 ~ ES-ATA-165 | urutonde rwa 65 ω-Transaminase, 50 mg buri kintu 65 * 50mg / ikintu, cyangwa ubundi bwinshi |
| Igikoresho cyo kwerekana (SynKit) | ES-ATA-6500 | urutonde rwa 65 ω-Transaminase, 1 mg buri kintu 65 * 1mg / ikintu |
Sub Substrate yihariye.
Guhitamo chiral ikomeye.
Impinduka nziza cyane.
Ibicuruzwa bike.
Conditions Imiterere yoroheje.
Yangiza ibidukikije.
Screen Kugenzura Enzyme bigomba gukorwa kubutaka bwihariye kubera umwihariko wa substrate, no kubona enzyme itera intego yibikorwa hamwe ningaruka nziza ya catalitiki.
➢ Ntuzigere uhura nibihe bikabije nka: ubushyuhe bwo hejuru, hejuru / hasi ya pH hamwe na organic solvent hamwe nibitekerezo byinshi.
➢ Mubisanzwe, sisitemu yogukora igomba kuba irimo substrate, igisubizo cya buffer, umuterankunga wa amino (nka acide amino na 1-fenyl etylamine) cyangwa reseptor (nka acide keto), coenzyme (PLP), cosolvent (nka DMSO).
➢ ATA igomba kongerwaho nyuma muri sisitemu yo kwitwara, nyuma ya pH nubushyuhe byahinduwe kumiterere.
Ubwoko bwose bwa ATA bufite uburyo bwiza bwo kwitwara neza, kuburyo buri kimwe muri byo kigomba gukomeza kwigwa kugiti cye.
Urugero rwa 1 (synthesis ya Sitagliptin, synthesis idasanzwe)(1):
Urugero rwa 2 (Mexiletine, Gukomatanya gukemura kinetic hamwe na synthesis ya asimmetric)(2):
1 Savile CK, Janey JM, Mundorff EC, n'abandi.Ubumenyi, 2010, 329 (16), 305-309.
2 Koszelewski D, Pressnitz D, Ibumba D, n'abandi.Inzandiko ngenga, 2009,11 (21): 4810-4812.
