Transaminase (ATA)
Enzymes: Ni catalysts za macromolecular biological, enzymes nyinshi ni poroteyine.
Transaminase: Ubwoko bwa enzymes zituma amino yimuka hagati ya aside amine na aside keto. Transaminase ni enzymes z'ingenzi mu mikorere y'uturemangingo tw'ibinyabuzima mu guhuza aside amine na aside keto.
Aminotransferase ishobora kugabanywamo ibyiciro bine hakurikijwe urukurikirane n'imiterere: Ⅰ, Ⅱ, Ⅲ na Ⅳ. ω-aminotransferase ziri muri transaminase zo mu cyiciro cya kabiri, zikunze gukoreshwa mu gutegura amine za chiral na aside amine zidasanzwe, nka aside β-amino.
ω-aminotransferase: Akenshi, ω-transaminase yerekeza ku bwoko bwa enzymes, zituma amoniya ihinduka nta aside α-amino nk'ikintu cyangwa umusaruro.
Uburyo bwo gukamura ibintu:
| Imisemburo | Kode y'igicuruzwa | Kode y'igicuruzwa |
| Ifu ya enzyme | ES-ATA-101~ ES-ATA-165 | seti ya 65 ω-Transaminases, 50 mg buri kimwe 65 ibintu * 50mg / ikintu, cyangwa ikindi gipimo |
| Agakoresho ko gusuzuma (SynKit) | ES-ATA-6500 | seti ya 65 ω-Transaminases, 1 mg buri kimwe 65 ibintu * 1mg / ikintu |
★ Uburyo bwinshi bwo gukoresha substrate.
★ Guhitamo gukomeye kw'abacuranzi.
★ Uburyo bwo guhindura ibintu bukoresha ubushobozi bwo hejuru.
★ Ibicuruzwa bike biva kuri ibyo.
★ Indwara zoroheje zo mu mutwe.
★ Irinda ibidukikije.
➢ Hakwiye gukorwa isuzuma rya enzyme ku bice bimwe na bimwe by’ingenzi bitewe n’imiterere yabyo, kandi hagakorwa enzyme ihindura imiterere yabyo ku bice bimwe na bimwe by’ingenzi.
➢ Ntukigere uhura n'ibintu bikomeye nko: ubushyuhe bwinshi, pH iri hejuru/hasi n'umusemburo w'ibinyabuzima ufite ubushyuhe bwinshi.
➢ Ubusanzwe, uburyo bwo gusubiza ibintu bugomba kuba burimo substrate, solution ya buffer, amino donor (nk'aside amine na 1-phenyl ethylamine) cyangwa receptor (nk'aside keto), coenzyme (PLP), cosolvent (nk'aside DMSO).
➢ ATA igomba kongerwaho nyuma muri sisitemu y'ibisubizo, nyuma y'uko pH n'ubushyuhe bihinduwe hakurikijwe imiterere y'ibisubizo.
➢ Ubwoko bwose bwa ATA bufite uburyo butandukanye bwo gusubiza ibintu neza, bityo buri kimwe muri byo kigomba kwigwaho ku giti cyacyo.
Urugero rwa 1 (gukora Sitagliptin, gukora asymmetric synthesis)(1):
Urugero rwa 2 (Mexiletine, Uruvange rw'ubushobozi bwa kinetic hamwe n'uburyo butangana)(2):
1 Savile CK, Janey JM, Mundorff EC, n'abandi. Siyansi, 2010, 329 (16), 305-309.
2 Koszelewski D, Pressnitz D, Ibumba D, n'abandi. Inzandiko ngenga, 2009,11 (21): 4810-4812.
