Foshokinase (PKase)
Ibisobanuro bigufi:
Ibyerekeye Phosphokinase
ES-PKase (Phosphokinase): itera ihererekanyabubasha ryamatsinda ya fosifate kuri ATP mubindi bikoresho.Ihindura kandi ihererekanyabubasha ryamatsinda ya fosifate kurindi nucleoside ya triphosifate rimwe na rimwe.Kinase nyinshi ikenera ibyuma bya ion kugirango bigire uruhare mubitekerezo (muri rusange Mg2 +).Hariho ubwoko 21 bwibicuruzwa bya enzyme ya PKase (Umubare nka ES-PKase101 ~ ES-PKase-121) byakozwe na SyncoZymes.
Ubwoko bwa catalitiki yerekana:
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E-Mail:lchen@syncozymes.com
| Enzymes | Kode y'ibicuruzwa | Ibisobanuro |
| Igikoresho cyo kwerekana (SynKit) | ES-PKase-101 ~ ES-PKase-121 | urutonde rwa 21 Ketoreductase, 1 mg buri kintu 21 * 1mg / ikintu |
Sub Substrate yihariye.
Guhitamo chiral ikomeye.
Guhindura byinshi.
Ibicuruzwa bike.
Conditions Imiterere yoroheje.
Yangiza ibidukikije.
➢ Mubisanzwe, sisitemu yogukora igomba kuba irimo substrate, igisubizo cya buffer, enzyme, ATP, Mg2+.
➢ PKase igomba kongerwaho nyuma muri sisitemu yo kwitwara, nyuma ya pH nubushyuhe byahinduwe kumiterere.
Urugero rwa 1 (Synthesis ya nicotinamide ribose fosifate kuva nicotinamide ribose)(1):
Icyitonderwa: ingero zikoreshwa hamwe nibisobanuro bigamije kwerekana urugero rwa porogaramu ya PKase kugirango byorohe byumvikane, kandi ntabwo bihuye na enzyme yihariye ya SyncoZymes.
Komeza imyaka 2 munsi -20 ℃.
Ntuzigere uhura nibihe bikabije nka: ubushyuhe bwo hejuru, hejuru / hasi ya pH hamwe nubushakashatsi bwimbitse.
1. Khan, Javed A., Indirimbo Xiang, na Liang Tong.Imiterere 15.8 (2007): 1005-1013.
