Phenylalanine dehydrogenase (PDH)
Ibisobanuro bigufi:
Ibyerekeye Phenylalanine dehydrogenase
ES-PDH: Icyiciro cya oxydeore.Imyitwarire myiza irashobora gutuma okiside yangiza umunyu wa L fenylalanine imbere ya NAD kugirango itange umunyu wa fenylpyruvate, kandi reaction irashobora guhinduka muguhindura umunyu wa aside amine uva mumunyu wa fenylpyruvate.Syncozymes yateje imbere ibintu 8 bya fenylalanine dehydrogenase (ifite numero ES-PDH-101 ~ ES-PDH-108).ES-PDH irashobora gukoreshwa mugutegura umunyu wa fenylalanine cyangwa umunyu wa aside amine uva mumunyu wa acide ya fenylpyruvic cyangwa ibikomoka.
Ubwoko bwa catalitiki yerekana:
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E-Mail:lchen@syncozymes.com
| Enzymes | Kode y'ibicuruzwa | Ibisobanuro |
| Ifu ya Enzyme | ES-PDH-101 ~ ES-PDH-108 | urutonde rwa 8 Nitrile Kugabanya, 50 mg buri kintu 8 * 50mg / ikintu, cyangwa ubundi bwinshi |
Sub Substrate yihariye.
Guhitamo chiral ikomeye.
Guhindura byinshi.
Ibicuruzwa bike.
Conditions Imiterere yoroheje.
Yangiza ibidukikije.
➢ Mubisanzwe, sisitemu yogukora igomba kuba irimo substrate, igisubizo cya buffer, enzyme, coenzyme (NAD (H)), sisitemu yo kuvugurura coenzyme (urugero: amonium ikora na dehydrogenase).
➢ Ubwoko bwose bwa ES-PDH bujyanye nuburyo bwiza bwo kwitwara neza, bushobora kwigwa kugiti cyawe nibikenewe.
ES ES-PDH igomba kongerwaho nyuma muri sisitemu yo gukomeza ibikorwa.
Urugero rwa 1 (Biosynthesis ya fenylalanine ikomoka kuri fenylpyruvate)(1):
Komeza imyaka 2 munsi -20 ℃.
Ntuzigere uhura nibihe bikabije nka: ubushyuhe bwo hejuru, hejuru / hasi ya pH hamwe nubushakashatsi bwimbitse.
1. Francesca Paradisi, Stuart Collins, n'abandi.Ikinyamakuru cya Biotechnologiya, 2007, 128, 408–411.
