Phenylalanine dehydrogenase (PDH)
Ibisobanuro bigufi:
Ku bijyanye na Phenylalanine dehydrogenase
ES-PDH: Ubwoko bwa oxidoreductase. Igisubizo cyiza gishobora gukurura deamination ya oxidative y'umunyu wa L phenylalanine mu gihe hari NAD kugira ngo ikore umunyu wa phenylpyruvate, kandi igisubizo gisubira inyuma gishobora gukurura ikorwa ry'umunyu wa aside amine ujyanye n'uwo munyu wa phenylpyruvate. Syncozymes yakoze ibintu 8 bya phenylalanine dehydrogenase (ifite inomero ES-PDH-101~ES-PDH-108). ES-PDH ishobora gukoreshwa mu gutegura umunyu wa phenylalanine cyangwa umunyu wa aside amine ujyanye n'uwo munyu wa aside phenylpyruvic cyangwa ibikomoka kuri wo.
Ubwoko bw'ingaruka za "catalytic reaction":
Telefoni igendanwa/Wechat/WhatsApp: +86-13681683526
Imeri:lchen@syncozymes.com
| Imisemburo | Kode y'igicuruzwa | Ibisobanuro |
| Ifu ya enzyme | ES-PDH-101~ ES-PDH-108 | seti ya 8 Nitrile Reductase, 50 mg buri kimwe 8 ibintu * 50mg / ikintu, cyangwa ikindi gipimo |
★ Uburyo bwinshi bwo gukoresha substrate.
★ Guhitamo gukomeye kw'abacuranzi.
★ Guhinduranya ibintu byinshi.
★ Ibicuruzwa bike biva kuri ibyo.
★ Indwara zoroheje zo mu mutwe.
★ Irinda ibidukikije.
➢ Ubusanzwe, sisitemu y'ibisubizo igomba kuba irimo substrate, solution ya buffer, enzyme, coenzyme (NAD (H)), sisitemu yo kuvugurura coenzyme (urugero: ammonium formate na formate dehydrogenase).
➢ Buri bwoko bwa ES-PDH bujyanye n'imiterere itandukanye myiza y'ibizamini, bushobora kwigwa ukwabwo mu gihe bibaye ngombwa.
➢ ES-PDH igomba kongerwaho nyuma muri sisitemu y'ibisubizo kugira ngo igikorwa gikomeze.
Urugero rwa 1 (Biosynthesis y'ibikomoka kuri phenylalanine biva muri phenylpyruvate derivatives)(1):
Bika ahantu hatagera ku gipimo cya -20°C imyaka 2.
Ntukigere uhura n'ibintu bikomeye nko: ubushyuhe bwinshi, pH iri hejuru/hasi ndetse n'ibinyabutabire byinshi.
1. Francesca Paradisi, Stuart Collins, n'abandi. Ikinyamakuru cy'ikoranabuhanga rya Biotechnology, 2007, 128, 408–411.
