SyncoZymes

ibicuruzwa

Okiside ya alcool (AOX)

Ibisobanuro bigufi:

Ibyerekeye okiside ya alcool

ES-AOXs: Iyi misemburo irashobora guhagarika okiside ya alcool yuzuye amavuta cyangwa aryl-alcool kugirango ibe aldehydes.Bakoresha ogisijeni ya molekile ariko ntibisaba cofactor yongeyeho hanze, na hydrogen peroxide yakozwe.

Hariho ubwoko 6 bwibicuruzwa bya alcool (Umubare nka ES-AOX-101 ~ ES-AOX-106) byakozwe na SyncoZymes.ES-AOX101 na ES-AOX102 bahitamo ibinyobwa bisindisha bya alifatique, ES-AOX103 ~ ES-AOX105 bahitamo ibinyobwa bisindisha, naho ES-AOX106 ni okiside ya cholesterol.SZ-AOX nigikoresho cyingirakamaro muguhagarika okiside ya alcool yibinure cyangwa aryl-alcool kugirango itange aldehydes.

Ubwoko bwa catalitiki yerekana:

AOX

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E-Mail:lchen@syncozymes.com


Ibicuruzwa birambuye

Ibicuruzwa

Amakuru y'ibicuruzwa:

Inzoga oxydease1
Enzymes Kode y'ibicuruzwa Ibisobanuro
Ifu ya Enzyme ES-AOX-101 ~ ES-AOX-105 urutonde rwa okiside 5 ya alcool, 50 mg buri kintu 5 * 50mg / ikintu, cyangwa ibindi byinshi
Igikoresho cyo kwerekana (SynKit) ES-AOX-500 urutonde rwa okiside 5 ya alcool, 50 mg buri kintu 5 * 50mg / ikintu, cyangwa ibindi byinshi

Ibyiza:

Sub Substrate yihariye.
Guhindura byinshi.
Ibicuruzwa bike.
Conditions Imiterere yoroheje.
Yangiza ibidukikije.

Amabwiriza yo gukoresha:

➢ Mubisanzwe, sisitemu yogukora igomba kuba irimo substrate, igisubizo cya buffer na ES-AOX, na ogisijeni igomba kuba ihari.
Ubwoko bwose bwa ES-AOXs bujyanye nuburyo bwiza bwo kwitwara neza, bushobora kwigwa kugiti cyawe.
Kwibanda cyane Substrate cyangwa ibicuruzwa hamwe bishobora kubuza ibikorwa bya ES-AOX.Ariko, kubuzwa birashobora koroherezwa mugice cyo kongeramo substrate.
Gukusanya H.2O2muri sisitemu bizaganisha kuri enzyme idakora, ishobora gukemurwa neza no gukoresha catalase.

Ingero zo gusaba:

Urugero rwa 1 (Oxidation ya aryl-alcool)(1):

Urugero rwa 1 (Oxidation ya aryl-alcool)

Urugero rwa 2 (Oxidation ya alcool ibinure)(2):

Urugero rwa 2 (Oxidation ya alcool ibinure)

Ububiko:

Komeza imyaka 2 munsi -20 ℃.

Icyitonderwa:

Ntuzigere uhura nibihe bikabije nka: ubushyuhe bwo hejuru, hejuru / hasi ya pH hamwe nubushakashatsi bwimbitse.

Reba:

1. Benen J AE, Sa 'nchez-Torres P, Wagemaker M JM, e tal.J Biol Chem, 1998, 273 (14): 7865-7872.
2. Mauersberger S, Drechsler H, Oehme G, e tal.Koresha Microbiol Biot, 1992, 37: 66-73.


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