Oxidase y'inzoga (AOX)
Ibisobanuro bigufi:
Ku bijyanye na oksidase y'inzoga
ES-AOXs: Izi enzymes zishobora gukurura ogisijeni ya alkoholi zirimo ibinure cyangwa aryl-alcohols kugira ngo zikore aldehydes. Zikoresha ogisijeni ya molekile ariko ntizikenera cofactor yongewemo inyuma, na peroxide ya hydrogen ikorwa.
Hari ubwoko butandatu bw'ibikomoka kuri okisidasi z'inzoga (Umubare nka ES-AOX-101~ES-AOX-106) byakozwe na SyncoZymes. ES-AOX101 na ES-AOX102 bikunda substrates za aliphatic alcohol, ES-AOX103~ ES-AOX105 bikunda substrates za aromatic alcohol, naho ES-AOX106 ikaba cholesterol oxidase. SZ-AOX ni igikoresho cy'ingirakamaro cyo gukurura okisidasi ya alcohol zirimo ibinure cyangwa aryl-alcohols kugira ngo ikore aldehydes.
Ubwoko bw'ingaruka za "catalytic reaction":
Telefoni igendanwa/Wechat/WhatsApp: +86-13681683526
Imeri:lchen@syncozymes.com
| Imisemburo | Kode y'igicuruzwa | Ibisobanuro |
| Ifu ya enzyme | ES-AOX-101~ ES-AOX-105 | seti ya oxidase 5 za alcool, 50 mg buri kimwe ibintu 5 * 50mg / ikintu, cyangwa ikindi gipimo |
| Agakoresho ko gusuzuma (SynKit) | ES-AOX-500 | seti ya oxidase 5 za alcool, 50 mg buri kimwe ibintu 5 * 50mg / ikintu, cyangwa ikindi gipimo |
★ Uburyo bwinshi bwo gukoresha substrate.
★ Guhinduranya ibintu byinshi.
★ Ibicuruzwa bike biva kuri ibyo.
★ Indwara zoroheje zo mu mutwe.
★ Irinda ibidukikije.
➢ Ubusanzwe, uburyo bwo gusubiza ibintu bugomba kuba burimo substrate, solution ya buffer na ES-AOX, kandi ogisijeni igomba kuba iriho.
➢ Ubwoko bwose bwa ES-AOXs bujyanye n'imiterere itandukanye myiza y'imikorere, bushobora kwigwa ukwabwo.
➢ Ubunini bwinshi bw'ibinyabutabire cyangwa ibikomoka ku bimera bishobora kubangamira imikorere ya ES-AOX. Ariko, ubu buryo bwo kubuza ibintu bushobora kugabanuka no kongeramo ibinyabutabire.
➢ Gukusanya H2O2muri sisitemu bizatuma enzyme idakora, ibyo bikaba byakemurwa neza hakoreshejwe catalase.
Urugero rwa 1 (Gushyira ogisijeni mu nzoga z'inzoga za aryl)(1):
Urugero rwa 2 (Gushyira ogisijeni mu binyabutabire birimo alcool)(2):
Bika ahantu hatagera ku gipimo cya -20°C imyaka 2.
Ntukigere uhura n'ibintu bikomeye nko: ubushyuhe bwinshi, pH iri hejuru/hasi ndetse n'ibinyabutabire byinshi.
1. Benen J AE, Sa 'nchez-Torres P, Wagemaker M JM, e tal. J Biol Chem, 1998, 273 (14): 7865-7872.
2. Mauersberger S, Drechsler H, Oehme G, e tal. Koresha Microbiol Biot, 1992, 37: 66-73.
