Nitrile hydratase (NHT)
Ibisobanuro bigufi:
Ku bijyanye na ES-NHTs
ES-NHTs: ubwoko bwa metalloenzyme, butuma nitriles zihinduka mu buryo bwihariye muri amide y'ibanze ijyanye nayo. Zikoreshwa cyane atari mu gukora aside amine, aside karbokisilike n'ibikomoka kuri zo gusa, ahubwo no mu gutegura imiti ya chiral. Hari ubwoko 24 bwa nitrile hydratases zakozwe na SyncoZymes (Umubare nka ES-NHT-101～ES-NHT-124).
Ubwoko bw'ingaruka za "catalytic reaction":
Telefoni igendanwa/Wechat/WhatsApp: +86-13681683526
Imeri:lchen@syncozymes.com
| Imisemburo | Kode y'igicuruzwa | Ibisobanuro |
| Ifu ya enzyme | ES-NHT-101~ ES-NHT-124 | seti ya Nitrile Hydratases 24, 50 mg buri kimwe 24 * 50mg / ikintu, cyangwa ikindi gipimo |
| Agakoresho ko gusuzuma (SynKit) | ES-NHT-2400 | seti ya 24 Nnitrile Hydratases, 1 mg buri kimwe 24 ibintu * 1mg / ikintu |
★ Uburyo bwinshi bwo gukoresha substrate.
★ Guhitamo gukomeye kw'abacuranzi.
★ Guhinduranya ibintu byinshi.
★ Ibicuruzwa bike biva kuri ibyo.
★ Indwara zoroheje zo mu mutwe.
★ Irinda ibidukikije.
➢ Ubusanzwe, sisitemu y'ibisubizo igomba kuba irimo substrate, umuti wa buffer (pH nziza y'ibisubizo) na ES-NHT.
➢ ES-NHT zose zishobora kugeragezwa muri sisitemu yo gusubiza yavuzwe haruguru cyangwa hakoreshejwe NHT Screening Kit (SynKit NHT).
➢ Ubwoko bwose bwa ES-NHTs bujyanye n'imiterere itandukanye myiza y'ibizamini bugomba kwigwa ukwabwo.
➢ Ubucucike bwinshi cyangwa ikintu gifite bishobora kubangamira imikorere ya ES-NHT. Ariko, ubu bucucike bushobora kugabanuka no kongeramo igice cy’ikintu, guhuzwa na amidase cyangwa gukuramo ikintu.
Urugero rwa 1 (guhuza na amidase)(1):
Urugero rwa 2(2):
Bika ahantu hatagera ku gipimo cya -20°C imyaka 2.
Ntukigere uhura n'ibintu bikomeye nko: ubushyuhe bwinshi, pH iri hejuru/hasi ndetse n'ibinyabutabire byinshi.
1 Vojtech V, Ludmila M, Alicja BV, e tal. J Mol Catal B-enzym, 2011, 71: 51-55.
2 Gary W. B, Thomas G, Christopher B. M, n'abandi. Tetrahedron lett, 2010, 51: 1639-1641.
