Leucine Dehydrogenase (LeuDH)
Ibisobanuro bigufi:
Ku bijyanye na Leucine dehydrogenase
Hari ubwoko bumwe bw'umusaruro wa enzyme ya LeuDH (Umubare nka ES-LeuDH-101) wakozwe na SyncoZymes. LeuDH ishobora gukurura amoniya igabanya aside 4-methylbutanoic (umunyu) kuri L-leucine cyangwa ogisijeni ya leucine kuri aside 4-methylbutanoic. ES-LeuDH ishobora kandi gukurura amino acide α-keto ifite imiterere isa kugira ngo itegure amino acide ijyanye nayo. Mu gihe cyo gukatalisa, NADH ni ngombwa nk'umuyoboro wa hydrogen.
Ubwoko bw'ingaruka za Leucine dehydrogenasecatalytic
Telefoni igendanwa/Wechat/WhatsApp: +86-13681683526
Imeri:lchen@syncozymes.com
| Imisemburo | Kode y'igicuruzwa | Ibisobanuro |
| Ifu ya enzyme | ES-LeuDH-101 | seti ya Monoamine Oxidase 1, 50 mg buri kimwe Ikintu 1 * 50mg / ikintu, cyangwa ikindi gipimo |
Uburyo bukurikira bwo gusubiza bushobora gukoreshwa mu kwifashisha:
1-10 mg/ml: Igice cy'ingenzi
10 mg/ml: Enzyme
10% (v/v): Cosolvent y'umwimerere (1)
100 mM: Fosfate buffer (pH7.0)
0.2 mg/ml: NAD+
100 mM: Ammonium formate
10 mg/ml: Formate dehydrogenase (FDH)
Shyira ingaruka ku gikoresho gipima ubushyuhe (urugero, 30oC, 150rpm) mu gihe cy'amasaha 24 kugeza kuri 48. Shyira kuri buri mvange kugira ngo poroteyine irusheho kuba nziza, aho supernatant ishobora gukoreshwa mu gusesengura TLC, HPLC cyangwa GC.
Icyitonderwa (1): Umusemburo w’ibinyabuzima (nka DMSO, methanol, acetonitrile, IPA, nibindi) ni uwo guhitamo, ukoreshwa mu bintu bidashonga.
