Epoxide hydrolase (EH)
Ibisobanuro bigufi:
Ku bijyanye na epoxide hydrolase
EH ni ether hydrolase itera hydrolysis ya stereoselective ya epoxides za racemic kugira ngo ikore 1, 2-diol na optique active epoxides, ishobora gukoreshwa mu gusubiza epoxides cyangwa gutegura chiral diols. Ikoreshwa cyane mu buvuzi no mu zindi nzego. Hari ubwoko butatu bw'ibicuruzwa bya epoxide hydrolases (Umubare nka ES-EH-101～ES-EH-103) byakozwe na SyncoZymes. ES-EH ni igikoresho cy'ingirakamaro cyo gutera imikorere ya chiral alcohols binyuze muri stereoselective hydrolysis ya aliphatic na aromatic epoxides.
Ubwoko bw'ingaruka za "catalytic reaction":
Telefoni igendanwa/Wechat/WhatsApp: +86-13681683526
Imeri:lchen@syncozymes.com
| Imisemburo | Kode y'igicuruzwa | Ibisobanuro |
| Ifu ya enzyme | ES-EH-101~ ES-EH-103 | seti ya Epoxide Hydrolases 3, 50 mg buri kimwe ibintu 3 * 50mg / ikintu, cyangwa ikindi gipimo |
| Agakoresho ko gusuzuma (SynKit) | ES-EH-300 | seti ya Epoxide Hydrolases 3, 1 mg buri kimwe Ibintu 3 * 1mg / ikintu |
★ Uburyo bwinshi bwo gukoresha substrate.
★ Guhitamo gukomeye kw'abacuranzi.
★ Guhinduranya ibintu byinshi.
★ Ibicuruzwa bike biva kuri ibyo.
★ Indwara zoroheje zo mu mutwe.
★ Irinda ibidukikije.
➢ Ubusanzwe, uburyo bwo gusubiza ibintu bugomba kuba burimo substrate, solution ya buffer (pH nziza yo gusubiza ibintu) na ES-EH.
➢ Ubwoko bwose bwa ES-EHs bujyanye n'imiterere itandukanye myiza y'ibizamini bugomba kwigwa ukwabwo.
➢ Ubucucike bwinshi cyangwa ikintu gifite ubucucike bushobora kubangamira imikorere ya ES-EH. Ariko, ubu bucucike bushobora kugabanuka no kongeramo ubucucike.
Urugero rwa 1(1):
Bika ahantu hatagera ku gipimo cya -20°C imyaka 2.
Ntukigere uhura n'ibintu bikomeye nko: ubushyuhe bwinshi, pH iri hejuru/hasi ndetse n'ibinyabutabire byinshi.
1. Kim YH, Lee I, Choi SH, e tal. J Mol Catal B-Enzym, 2013, 89: 48-51.
