Fosforasi ya Nucloside (NP)

Ibisobanuro bigufi:

Ku bijyanye na fosifori ya nucleoside

Hari ubwoko butatu bw'ibikomoka kuri enzyme ya NP (Umubare nka ES-NP-101 ~ ES-NP-103) byakozwe na SyncoZymes. ES-NP-101 ni purine nucleoside phosphorylase, ES-NP-102 na ES-NP-103 ni pyrimidine nucleoside phosphorylase. Nucleosidephosphorylase ishobora gutandukanya nucleosides mo bases na pentose phosphate. Nucleoside phosphorylase ishobora kugabanywamo purine nucleoside phosphorylase na pyrimidine nucleoside phosphorylase hakurikijwe ibyo bases za nucleoside zikunda. Purine nucleoside phosphorylase ishobora gusya adenosine kuri adenine, inosine kuri hypoxanthine, guanosine kuri guanine, no gukora ribose phosphate icyarimwe. Pyrimidine nucleoside phosphorylase ishobora gusya uridine kuri uracil no gukora ribose phosphate.

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Ibisobanuro birambuye ku gicuruzwa

Ibirango by'ibicuruzwa

Ubwoko bw'inyito ya phosphorylse ya Nucloside:

Amakuru ku bicuruzwa:

Imisemburo	Kode y'igicuruzwa	Ibisobanuro
Ifu ya enzyme	ES-NP-101~ ES-NP-103	seti ya Nuclosidephosphorylse 3, 50 mg buri kimwe ibintu 3 * 50mg / ikintu, cyangwa ikindi gipimo

Uburyo bwo gusuzuma ingaruka za Nucloside phosphorylse:

Uburyo bukurikira bwo gusubiza bushobora gukoreshwa mu kwifashisha:
Igice cya 1-10 mg/ml
Enzyme ya 10 mg/ml
Fosfeti ya 100 mM (pH7.0)
Shyira ingaruka ku gikoresho gipima ubushyuhe (urugero, 30oC, 150rpm) mu gihe cy'amasaha 12-24. Shyira kuri buri mvange kugira ngo poroteyine irusheho kuba nziza, aho supernatant ishobora gukoreshwa mu gusesengura TLC, HPLC cyangwa GC.