Nitrilase (NIT)
Ibisobanuro bigufi:
Ibyerekeye Nitrilase
ES-NITs: Guhindura itsinda rya cyanide mu itsinda rya carboxyl, kugira ngo hategurwe aside carboxylic, ikoreshwa cyane mu buvuzi, kurengera ibidukikije, inganda z'imiti, inyongeramusaruro z'ibiribwa, imyenda, umusaruro w'ubuhinzi n'ibindi bikorwa.
Hari ubwoko 40 bw'ibicuruzwa bya Nitrilase (NIT) (Umubare nka ES-NIT-101~ES-NIT-140) byakozwe na SyncoZymes. ES-NIT ni igikoresho cy'ingirakamaro cyo gushishikariza ikorwa rya aside carboxylic chiral binyuze muri hydrolysis ya regio- na stereoselective ya nitriles nyinshi za aliphatic na aromatic.
Ubwoko bw'ingaruka za "catalytic reaction":
Telefoni igendanwa/Wechat/WhatsApp: +86-13681683526
Imeri:lchen@syncozymes.com
| Imisemburo | Kode y'igicuruzwa | Ibisobanuro |
| Ifu ya enzyme | ES-NIT-101~ ES-NIT-140 | seti ya 40 Nitrilase, 50 mg buri kimwe 40 ibintu * 50mg / ikintu, cyangwa ikindi gipimo |
| Agakoresho ko gusuzuma (SynKit) | ES-NIT-4000 | seti ya Nitrilase 40, 1 mg buri kimwe Ibintu 40 * 1mg / ikintu |
★ Uburyo bwinshi bwo gukoresha substrate.
★ Guhinduranya ibintu byinshi.
★ Ibicuruzwa bike biva kuri ibyo.
★ Indwara zoroheje zo mu mutwe.
★ Irinda ibidukikije.
➢ Ubusanzwe, uburyo bwo gusubiza ibintu bugomba kuba burimo substrate, solution ya buffer (pH nziza yo gusubiza ibintu) na ES-NIT.
➢ ES-NIT zose zishobora kugeragezwa muri sisitemu yo gusubiza yavuzwe haruguru cyangwa hakoreshejwe NIT Screening Kit (SynKit NIT).
➢ Ubwoko bwose bwa ES-NITs bujyanye n'imiterere itandukanye myiza y'ibizamini bugomba kwigwa ukwabwo.
➢ Ubucucike bwinshi cyangwa ikintu gifite ubucucike bushobora kubuza imikorere ya ES-NIT. Ariko, ubu bucucike bushobora kugabanuka no kongeramo ubucucike.
Urugero rwa 1 (Gukora aside nikotine)(1):
Urugero rwa 2 (Gukora aside ya (R)-(-)-mandelic)(2):
Urugero rwa 3 (Gukora baclofen)(3):
Urugero rwa 4 (Gukora aside glycolic)(4):
Bika ahantu hatagera ku gipimo cya -20°C imyaka 2.
Ntukigere uhura n'ibintu bikomeye nko: ubushyuhe bwinshi, pH iri hejuru/hasi ndetse n'ibinyabutabire byinshi.
1 Mathew CD, Nagasawa T, Kobayashi M, etal. Appl. Environ. Microbiol, 1988, 54 (4): 1030.
2 Yamamoto K, Oishi K, Fujimatsu I, e tal. Porogaramu. Ibidukikije. Microbiol, 1991, 57 (10): 3028.
3 Xu MZ, Ren J, Gong JS, e tal. Chin. J. Biotechnol, 2013, 29 (1): 31.
4 Wu S, Fogiel AJ, Petrillo KL, e tal. Ibinyabuzima. Bioeng., 2008, 99 (3): 717.
