Hydroaminase (HAM)
Enzymes | Kode y'ibicuruzwa | Ibisobanuro |
Ifu ya Enzyme | ES-HAM-101 ~ ES-HAM-102 | urutonde rwa 2 Nuclosidephosphoryalse, 50 mg buri kintu 2 * 50mg / ikintu, cyangwa ubundi bwinshi |
Sisitemu ikurikira irashobora gukoreshwa mubisobanuro:
1-10 mg / ml: Substrate
10 mg / ml: Enzyme
10% (v / v): Cosolvent organique (1)
10% (v / v): Amoniya
Hindura pH kuri 9.0 mbere yo kongeramo enzyme, na enzyme igomba kongerwamo amaherezo.Ongeramo reaction kumashanyarazi ahoraho (urugero, 30oC, 150rpm) mumasaha 24 ~ 48.Centrifuge buri mvange kugirango igabanye poroteyine, izo ndengakamere zirashobora gukoreshwa muri TLC 、 HPLC cyangwa GC.
Icyitonderwa (1): Solvent organic (nka DMSO, methanol, acetonitrile, IPA, nibindi) irahitamo, ikoreshwa kubutaka budashonga.
Andika ubutumwa bwawe hano hanyuma utwohereze