SyncoZymes

ibicuruzwa

Hydroaminase (HAM)

Ibisobanuro bigufi:

Ibyerekeye Hydroaminase

Hariho ubwoko 2 bwibicuruzwa bya HAM enzyme (Umubare nka ES-HAM-101 ~ ES-HAM-102) byakozwe na SyncoZymes.HAM irashobora guhagarika ammoniation ya acide enoic cyangwa ibiyikomokaho kugirango ikore aside chiral amino.HAM irashobora gukoreshwa mugushushanya chiral amino acide (cyangwa chiral amine) ivuye muri acide enoic (cyangwa alkenes).Umuterankunga wa Amoniya, nk'amazi ya amoniya cyangwa umunyu wa amonium, birakenewe.

Ubwoko bwa Hydroaminase catalitike yubwoko

Hydroaminase HAM2

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E-Mail:lchen@syncozymes.com


Ibicuruzwa birambuye

Ibicuruzwa

Amakuru y'ibicuruzwa:

Hydroaminase HAM
Enzymes Kode y'ibicuruzwa Ibisobanuro
Ifu ya Enzyme ES-HAM-101 ~ ES-HAM-102 urutonde rwa 2 Nuclosidephosphoryalse, 50 mg buri kintu 2 * 50mg / ikintu, cyangwa ubundi bwinshi

Hydroaminase yerekana uburyo bwo kubyitwaramo:

Sisitemu ikurikira irashobora gukoreshwa mubisobanuro:
1-10 mg / ml: Substrate
10 mg / ml: Enzyme
10% (v / v): Cosolvent organique (1)
10% (v / v): Amoniya
Hindura pH kuri 9.0 mbere yo kongeramo enzyme, na enzyme igomba kongerwamo amaherezo.Ongeramo reaction kumashanyarazi ahoraho (urugero, 30oC, 150rpm) mumasaha 24 ~ 48.Centrifuge buri mvange kugirango igabanye poroteyine, izo ndengakamere zirashobora gukoreshwa muri TLC 、 HPLC cyangwa GC.

Icyitonderwa (1): Solvent organic (nka DMSO, methanol, acetonitrile, IPA, nibindi) irahitamo, ikoreshwa kubutaka budashonga.


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