Hydroaminase (HAM)
Ibisobanuro bigufi:
Ku bijyanye na Hydroaminase
Hari ubwoko bubiri bw'umusaruro wa enzyme ya HAM (Umubare nka ES-HAM-101~ ES-HAM-102) wakozwe na SyncoZymes. HAM ishobora gukurura aside enoic cyangwa ibiyikomokaho kugira ngo ikore aside chiral amine. HAM ishobora gukoreshwa mu gukora aside chiral amine (cyangwa amine chiral) iva muri aside enoic (cyangwa alkenes). Umuntu utanga amino, nk'amazi ya amonia cyangwa umunyu wa amoniya, ni ngombwa.
Ubwoko bw'ingaruka za Hydroaminase catalytic
Telefoni igendanwa/Wechat/WhatsApp: +86-13681683526
Imeri:lchen@syncozymes.com
| Imisemburo | Kode y'igicuruzwa | Ibisobanuro |
| Ifu ya enzyme | ES-HAM-101~ ES-HAM-102 | seti ya Nuclosidephosphorylse 2, 50 mg buri kimwe 2 ibintu * 50mg / ikintu, cyangwa ikindi gipimo |
Uburyo bukurikira bwo gusubiza bushobora gukoreshwa mu kwifashisha:
1-10 mg/ml: Igice cy'ingenzi
10 mg/ml: Enzyme
10% (v/v): Cosolvent y'umwimerere (1)
10% (v/v): Amoniya
Hindura pH kuri 9.0 mbere yo kongeramo enzyme, hanyuma enzyme igomba kongerwamo amaherezo. Shyira ingaruka ku gikoresho gipima ubushyuhe (urugero, 30oC, 150rpm) mu masaha 24-48. Shyira kuri buri mvange kugira ngo poroteyine irusheho kuba nziza, aho supernatant ishobora gukoreshwa mu gusesengura TLC, HPLC cyangwa GC.
Icyitonderwa (1): Umusemburo w’ibinyabuzima (nka DMSO, methanol, acetonitrile, IPA, nibindi) ni uwo guhitamo, ukoreshwa mu bintu bidashonga.
