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SyncoZymes

ibicuruzwa

Ishusho ya Esterase & Lipase (PLE & CALB)
  • Esterase na Lipase (PLE na CALB)
  • Esterase na Lipase (PLE na CALB)

Esterase na Lipase (PLE na CALB)

Ibisobanuro bigufi:

Ku bijyanye na Esterase na Lipase

ES-PLE: Ubwoko bwa hydrolases zituma habaho kandi zigacika burundu imigozi ya ester. Zishobora kugira uruhare mu buryo butandukanye bwo gukora ibintu bya shimi, nko gupima, guhindura imiterere y’ibinyabutabire mu mubiri no gupima imiterere y’ibinyabutabire. Zikoreshwa cyane mu nzego nyinshi nko gutunganya ibiribwa, inganda zikora imiti, ubuvuzi bw’ibinyabuzima, ubuvuzi bw’ibinyabutabire no kuvura ibidukikije.

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Imeri:lchen@syncozymes.com


Ibisobanuro birambuye ku gicuruzwa

Ibirango by'ibicuruzwa

Ku bijyanye na Esterase na Lipase:

Hari ubwoko 26 bw'ibicuruzwa bya enzyme ya PLE (Umubare nka ES-PLE-101 ~ ES-PLE-126) byakozwe na SyncoZymes. ES-PLE ishobora gukoreshwa mu gukurura hydrolysis y'ibinyabutabire bya aliphatic na ester, cyangwa regioselective na stereoselective resolution kugira ngo hakorwe aside chiral n'ibikomoka kuri zo.

Ubwoko bw'ingaruka za "catalytic reaction":

Esterase na Lipase (PLE na CALB)
Esterase na Lipase (PLE na CALB)1

Ibyiza:

★ Uburyo bwinshi bwo gukoresha substrate.
★ Guhitamo gukomeye kw'abacuranzi.
★ Guhinduranya ibintu byinshi.
★ Ibicuruzwa bike biva kuri ibyo.
★ Indwara zoroheje zo mu mutwe.
★ Irinda ibidukikije.

Amabwiriza yo gukoresha:

➢ Ubusanzwe, sisitemu yo gusubiza ibintu igomba kuba irimo substrate, umuti wa buffer na ES-PLE. Gupima ES-PLE zimwe na zimwe bikorwa mu cyiciro cy’ibinyabuzima.
➢ Ubwoko bwose bwa ES-PLEs bujyanye n'imiterere itandukanye myiza y'ingaruka ziterwa n ...

Ingero z'Ishyirwa mu Bikorwa:

Urugero rwa 1 (Biosynthesis ya Pregabalin Intermediate)(1):

kalbu1

Urugero rwa 2(2):

kalb2

Urugero rwa 3(3):

kalbu3

Urugero rwa 4(4):

kalbu5

Ububiko:

Bika ahantu hatagera ku gipimo cya -20°C imyaka 2.

Icyitonderwa:

Ntukigere uhura n'ibintu bikomeye nko: ubushyuhe bwinshi, pH iri hejuru/hasi.

Amareferensi:

1. Xu FX, Chen SY, Xu G, e tal. Appl. Biotechnol Bioproc E, 1988, 54 (4): 1030.
2. Huang, FC, Lee, LF, Mittal, RSD e tal. J. Am. Chem.Soc, 1975, 97, 4144.
3. Kielbasinski, P., Goralczyk, P., Mikolajczyk, M., e tal. Synlett, 1994, 127.
4. Gais, HJ, Griebel, C., Buschmann, H. Tetrahedron: Asimmetry 2000, 11, 917

  • Ibanjirije iyi:
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