Esterase & Lipase (PLE & CALB)
Hariho ubwoko 26 bwibicuruzwa bya PLE enzyme (Umubare nka ES-PLE-101 ～ ES-PLE-126) byakozwe na SyncoZymes.ES-PLE irashobora gukoreshwa kuri hydrolysis ya alifatique na ester ivanze, cyangwa regioselective na stereoselective reaction yo guhuza acide chiral nibiyikomokaho.
Ubwoko bwa catalitiki yerekana:
Sub Substrate yihariye.
Guhitamo chiral ikomeye.
Guhindura byinshi.
Ibicuruzwa bike.
Conditions Imiterere yoroheje.
Yangiza ibidukikije.
➢ Mubisanzwe, sisitemu yogukora igomba gushiramo substrate, igisubizo cya buffer na ES-PLE.Esterification ya ES-PLEs ikorwa mugice kama.
Types Ubwoko bwose bwa ES-PLEs bujyanye nuburyo bwiza bwo kwitwara bugomba kwigwa kugiti cye.
Urugero rwa 1 (Biosynthesis ya Pregabalin Hagati)(1):
Urugero 2(2):
Urugero 3(3):
Urugero 4(4):
Komeza imyaka 2 munsi -20 ℃.
Ntuzigere uhura nibintu bikabije nka: ubushyuhe bwo hejuru, hejuru / hasi pH.
1. Xu FX, Chen SY, Xu G, e tal.Porogaramu.Biotechnol Bioproc E, 1988, 54 (4): 1030.
2. Huang, FC, Lee, LF, Mittal, RSD e tal.J. Am.Chem.Soc, 1975, 97, 4144.
3. Kielbasinski, P., Goralczyk, P., Mikolajczyk, M., e tal.Synlett, 1994, 127.
4. Gais, HJ, Griebel, C., Buschmann, H. Tetrahedron: Asimmetry 2000, 11, 917
