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SyncoZymes

ibicuruzwa

Ishusho ya Ene reductase (ERED)
  • Ene reductase (ERED)

Ene reductase (ERED)

Ibisobanuro bigufi:

Ku bijyanye na ene reductase

ES-ERED: Ziri mu muryango w'imisemburo ishaje y'umuhondo igabanya C=C mu buryo bwihariye mu bintu α, β-unsaturated hamwe n'amatsinda akura electron. Zikoreshwa cyane mu bushakashatsi ku bintu bikomoka kuri aside amine, ibintu bikora ku buzima, imiti ikoreshwa mu buvuzi, ibirungo n'ibice bya chiral.

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Imeri:lchen@syncozymes.com


Ibisobanuro birambuye ku gicuruzwa

Ibirango by'ibicuruzwa

Ku bijyanye na ene reductase:

ES-ERED zitera imbaraga ubwoko butandukanye bwa substrate kubera ubwoko bwinshi bwa substrate. Muri rusange, C=C y'ibintu bya α, β-unsaturated hamwe n'amatsinda akura electron (harimo ketone aldehyde, amatsinda ya nitro, aside carboxylic, esters, anhydride, lactones, imines, nibindi) bigabanuka byoroshye na ES-EREDs, ariko imigozi ibiri idakora ntabwo ikorwa.

Hari ubwoko 46 bw'ibicuruzwa bya enzyme ya ERED (Umubare nka ES-ERED-101~ES-ERED-146) byakozwe na SyncoZymes.

Uburyo bwo gukamura ibintu:

Ene reductase ERED1

Amakuru ku bicuruzwa:

Ene reductase ERED2
Imisemburo Kode y'igicuruzwa Ibisobanuro
Ifu ya enzyme ES-ERED-101~ ES-ERED-146 seti ya 46 Ene Reductases, 50 mg buri kimwe 46 ibintu * 50mg / ikintu, cyangwa ikindi gipimo
Agakoresho ko gusuzuma (SynKit) ES-ERED-4600 seti ya 46 Ene Reductases, 50 mg buri kimwe 46 ibintu * 50mg / ikintu, cyangwa ikindi gipimo

Ibyiza:

★ Uburyo bwinshi bwo gukoresha substrate.
★ Guhitamo gukomeye kw'abacuranzi.
★ Guhinduranya ibintu byinshi.
★ Ibicuruzwa bike biva kuri ibyo.
★ Indwara zoroheje zo mu mutwe.
★ Irinda ibidukikije.
★ Umutekano mwinshi.

Amabwiriza yo gukoresha:

➢ Ubusanzwe, sisitemu y'ibisubizo igomba kuba irimo substrate, umuti w'ibisubizo (pH nziza y'ibisubizo), coenzymes (NAD (H) cyangwa NADP (H) ), sisitemu yo kuvugurura coenzymes (urugero: glucose na glucose dehydrogenase) na ES-ERED.
➢ ES-ERED zose zishobora kugeragezwa muri sisitemu yo gusubiza yavuzwe haruguru cyangwa hakoreshejwe ERED Screening Kit (SynKit ERED).
➢ Ubwoko bwose bwa ES-EREDs bujyanye n'imiterere myiza y'uburyo butandukanye bwo gusubiza bugomba kwigwa ukwabwo.
➢ Ubunini bwinshi bw'ibinyabutabire cyangwa ibikomoka ku bimera bishobora kubangamira imikorere ya ES-ERED. Ariko, ubu buryo bwo kubuza ibintu bushobora kugabanuka no kongeramo ibinyabutabire.

Ingero z'Ishyirwa mu Bikorwa:

Urugero rwa 1 (α,β-aldehydes cyangwa ketones zidafite ubushyuhe)(1):

Ene reductase ERED3

Urugero rwa 2 (aside α,β-unsaturated carboxylic n'ibikomoka kuri zo)(2):

Ene reductase ERED4

Ububiko:

Bika ahantu hatagera ku gipimo cya -20°C imyaka 2.

Icyitonderwa:

Ntukigere uhura n'ibintu bikomeye nko: ubushyuhe bwinshi, pH iri hejuru/hasi ndetse n'ibinyabutabire byinshi.

Amareferensi:

1. Lucídio C, Fardelone J, Augusto R, e tal. J.Mol.Catal.B: Enzym., 2004, 29: 41-45.
2. Stueckler C, Hall M, Ehammer H, n'abandi. .Org.Lett, 2007, 9(26): 5409-5411.

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