Amidase (AMD)
Imisemburo:Ni katalysts za macromolecular biological, imisemburo myinshi ni poroteyine
Amidase:Koroshya uburyo bwo gukamura amide zitandukanye zo mu bwoko bwa aliphatic na aromatic amide zikomoka ku binyabuzima n'iz'amahanga binyuze mu kwimura itsinda rya acyl mu mazi hamwe n'ikorwa rya aside free na ammonia. Aside hydroxamic n'izindi aside organic zikoreshwa cyane nk'imiti kuko ari zo zigize ibintu bikura, antibiyotike n'ibibuza kanseri. Amide zishobora kugabanywamo aside R na S bitewe n'uburyo catalyst stereoselectivity ikora.
Uretse gukurura hydrolysis ya amides, amidase ishobora no gukurura acyl transfer reactions mu gihe hari co-substrates nka hydroxylamine.
Amidasi ifite amasoko atandukanye ifite imiterere itandukanye ya substrate, zimwe muri zo zishobora gusa guhisha amide aromatike, izindi zishobora gusa guhisha amide aliphatic, naho izindi zigashya α-cyangwa ω-amino amide. Amide nyinshi zifite imikorere myiza ya catalytic gusa kuri amide aromatike cyangwa simple aromatike, ariko kuri aromatike igoye, amide heterocyclic, cyane cyane amide zifite ortho substituents, muri rusange ziba nke mu mikorere yazo (enzyme nke gusa ni zo zigaragaza ingaruka nziza za catalytic).
Uburyo bwo gukamura ibintu:
| Imisemburo | Kode y'igicuruzwa | Kode y'igicuruzwa |
| Ifu ya enzyme | ES-AMD-101~ ES-AMD-119 | seti ya amidase 19, 50 mg buri kimwe ibintu 19 * 50mg / ikintu, cyangwa ikindi gipimo |
| Agakoresho ko gusuzuma (SynKit) | ES-AMD-1900 | seti ya amidase 19, 1 mg buri kimwe Ibintu 19 * 1mg / ikintu |
★ Uburyo bwinshi bwo gukoresha substrate.
★ Guhitamo gukomeye kw'abacuranzi.
★ Uburyo bwo guhindura ibintu bukoresha ubushobozi bwo hejuru.
★ Ibicuruzwa bike biva kuri ibyo.
★ Indwara zoroheje zo mu mutwe.
★ Irinda ibidukikije.
➢ Hakwiye gukorwa isuzuma rya enzyme ku bice bimwe na bimwe by’ingenzi bitewe n’imiterere yabyo, kandi hagakorwa enzyme ihindura imiterere yabyo ku bice bimwe na bimwe by’ingenzi.
➢ Ntukigere uhura n'ibintu bikomeye nko: ubushyuhe bwinshi, pH iri hejuru/hasi n'umusemburo w'ibinyabuzima ufite ubushyuhe bwinshi.
➢ Ubusanzwe, sisitemu y'ibisubizo igomba kuba irimo substrate, umuti wa buffer (pH nziza ya enzyme). Co-substrates nka hydroxylamine igomba kuba iri muri sisitemu y'ibisubizo bya acyl transfer.
➢ AMD igomba kongerwaho nyuma muri sisitemu y'ibisubizo ifite pH n'ubushyuhe byiza.
➢ Ubwoko bwose bwa AMD bufite uburyo butandukanye bwo gukora neza, bityo buri kimwe muri byo kigomba kwigwaho ku giti cyacyo.
Urugero rwa 1(1):
Igikorwa cya Hydrolysis ku bice bitandukanye bya Amide
| Inzu ntoya | Igikorwa cyihariye μmols iminota-1mg-1 | Inzu ntoya | Igikorwa cyihariye μmols iminota-1mg-1 |
| Asetamide | 3.8 | ο-OH benzamide | 1.4 |
| Propionamide | 3.9 | p-OH benzamide | 1.2 |
| Lactamide | 12.8 | ο-NH2benzamide | 1.0 |
| Butyramide | 11.9 | p-NH2benzamide | 0.8 |
| Isobutiramide | 26.2 | ο-Toluamide | 0.3 |
| Pentanamide | 22.0 | p-Toluamide | 8.1 |
| Hexanamide | 6.4 | Nikotinamide | 1.7 |
| Cyclohexanamide | 19.5 | Isonicotinamide | 1.8 |
| Acrylamide | 10.2 | Picolinamide | 2.1 |
| Metacrylamide | 3.5 | 3-Phenylpropionamide | 7.6 |
| Prolinamide | 3.4 | Indol-3-acetamide | 1.9 |
| Benzamide | 6.8 |
Uburyo bwo gukora bwakozwe mu gisubizo cya sodium phosphate buffer cya 50mM, pH 7.5, kuri 70 ℃.
| Amides | Hydroxylamine | Hydrazine |
| Asetamide | 8.4 | 1.4 |
| Propionamide | 18.4 | 3.0 |
| Isobutiramide | 25.0 | 22.7 |
| Benzamide | 9.2 | 6.1 |
Uburyo bwo gukora bwakozwe mu gisubizo cya sodium phosphate buffer cya 50mM, pH 7.5, kuri 70 ℃.
Ingano y'ibisubizo bifitanye isano: amides, 100 mM (benzamide, 10 mM); hydroxylamine na hydrazine, 400 mM; enzyme 0.9 μM.
Urugero rwa 2(2):
Urugero rwa 3(3):
1. D'Abusco AS, Ammendola S., n'abandi. Extremophiles, 2001, 5: 183-192.
2. Guo FM, Wu JP, Yang LR, n'abandi. Ubushakashatsi ku binyabuzima, 2015, 50 (8): 1400-1404.
3. Zheng RC, Jin JQ, Wu ZM, n'abandi. Ubutabire bw'ibinyabuzima, 2017, Biboneka kuri interineti 7.
