Okiside ya alcool (AOX)
Enzymes | Kode y'ibicuruzwa | Ibisobanuro |
Ifu ya Enzyme | ES-AOX-101 ~ ES-AOX-105 | urutonde rwa okiside 5 ya alcool, 50 mg buri kintu 5 * 50mg / ikintu, cyangwa ibindi byinshi |
Igikoresho cyo kwerekana (SynKit) | ES-AOX-500 | urutonde rwa okiside 5 ya alcool, 50 mg buri kintu 5 * 50mg / ikintu, cyangwa ibindi byinshi |
Sub Substrate yihariye.
Guhindura byinshi.
Ibicuruzwa bike.
Conditions Imiterere yoroheje.
Yangiza ibidukikije.
➢ Mubisanzwe, sisitemu yogukora igomba kuba irimo substrate, igisubizo cya buffer na ES-AOX, na ogisijeni igomba kuba ihari.
Ubwoko bwose bwa ES-AOXs bujyanye nuburyo bwiza bwo kwitwara neza, bushobora kwigwa kugiti cyawe.
Kwibanda cyane Substrate cyangwa ibicuruzwa hamwe bishobora kubuza ibikorwa bya ES-AOX.Ariko, kubuzwa birashobora koroherezwa mugice cyo kongeramo substrate.
Gukusanya H.2O2muri sisitemu bizaganisha kuri enzyme idakora, ishobora gukemurwa neza no gukoresha catalase.
Urugero rwa 1 (Oxidation ya aryl-alcool)(1):
Urugero rwa 2 (Oxidation ya alcool ibinure)(2):
Komeza imyaka 2 munsi -20 ℃.
Ntuzigere uhura nibihe bikabije nka: ubushyuhe bwo hejuru, hejuru / hasi ya pH hamwe nubushakashatsi bwimbitse.
1. Benen J AE, Sa 'nchez-Torres P, Wagemaker M JM, e tal.J Biol Chem, 1998, 273 (14): 7865-7872.
2. Mauersberger S, Drechsler H, Oehme G, e tal.Koresha Microbiol Biot, 1992, 37: 66-73.