Research Express | Ubushakashatsi bwa Kaminuza ya Tsinghua bwerekanye ko NMN ishobora kuvura ububyimbirwe

Gukora Macrophage ni uburyo butera indwara butera ububyimbirwe budakira mu mubiri, ariko gukora macrophage bidashira bishobora gutera ububyimbirwe budakira n'indwara nko kudakira insuline ndetse n'indwara zikomeye nka atherosclerosis. PGE 2, ihuza uburyo ububyimbirwe buterwa n'ububyimbirwe, ikorwa muri aside arachidonic na cyclooxygenases (COX-1 na COX-2). COX-1 na COX-2 ni byo bintu by'ingenzi birwanya ububyimbirwe kandi bishobora kubuzwa n'imiti irwanya ububyimbirwe itari steroide (NSAIDs).

Gukoresha imiti igabanya ubukana bw'indwara zo mu nda (NSAIDs) bishobora gutera ingaruka mbi nyinshi, nko kuva amaraso mu gifu. Kubwibyo, ni ngombwa cyane gushaka umuti karemano wizewe wo kuvura ububyimbirwe.

Vuba aha, itsinda ry’abashakashatsi bo muri Kaminuza ya Tsinghua ryavuje NMN ku macrophages y’imbeba, kandi ryagaragaje binyuze mu bushakashatsi ko NMN ishobora kugabanya ukwiyongera kwa poroteyine zifitanye isano n’ububyimbirwe n’ibikomoka kuri metabolike, no kubuza uburyo macrophages ikora ububyimbirwe.

Ububyimbirwe buhindura urugero rw'ibikomoka ku mikorere y'umubiri muri macrophages
Ubwa mbere, itsinda ry’ubushakashatsi ryakoresheje macrophages kugira ngo ikore ububyimbirwe binyuze muri lipopolysaccharide (LPS), hanyuma basesengura ingano y’ibikomoka kuri macrophages mu gihe cyo kubyimbirwa. Igipimo cya metabolite 99 cyariyongereye kandi metabolite 105 zaragabanutse muri molekile 458 zabonetse mbere na nyuma yo kubyimbirwa, kandi igipimo cya NAD+ giherekeza ububyimbirwe nacyo cyaragabanutse.

(ISHUSHO YA 1)

NMN yongera urugero rwa NAD kandi ikagabanya ububyimbirwe bwa macrophage
Hanyuma itsinda ry’ubushakashatsi ryavuje macrophages hifashishijwe LPS, ibi bikaba bitera indwara yo kubyimba, IL-6 na IL-1β, cytokines zitera ububyimbirwe zikora nk'ibimenyetso by'ububyimbirwe. Nyuma yo kuvura ububyimbirwe bwa macrophages buterwa na NMN, byagaragaye ko urugero rwa NAD mu ngirabuzima fatizo rwiyongereye kandi imiterere ya mRNA ya IL-6 na IL-1β iragabanuka. Ubushakashatsi bwagaragaje ko NMN yongereye urugero rwa NAD kandi igagabanya ububyimbirwe bwa macrophages buterwa na LPS.

(Ishusho ya 2)

(Ishusho ya 3)

NMN igabanya urugero rwa poroteyine ziterwa n'ububyimbirwe
Mu kuvura indwara ya NMN, byagaragaye ko poroteyine zijyanye no kubyimba nka RELL1, PTGS2, FGA, FGB na igkv12-44 zagabanutse mu turemangingo, ibyo bikaba byaragaragaje ko NMN yagabanyije imikorere ya poroteyine zijyanye no kubyimba.

(Ishusho ya 4)

NMN igabanya ikoreshwa rya poroteyine zigamije NSAIDS
Igerageza rya nyuma ryagaragaje ko NMN yagabanyije urugero rwa PGE2 mu turemangingo twa RAW264.7 twakozwe na LPS binyuze mu kugabanya urugero rw'imikorere ya COX-2, bityo ikagabanya imikorere ya COX2 kandi ikabuza ububyimbirwe buterwa na LPS.

(Ishusho ya 6)

Umwanzuro, kongerera NMN bishobora kuvura neza ububyimbirwe budakira ku mbeba, kandi uburyo bwo kuvura ububyimbirwe ku bantu buracyakeneye kwemezwa n'ibizamini by'ubuvuzi. Ahari NMN izaba isimbura NSAIDS mu gihe cya vuba.

ingero:
1.Liu J, Zong Z, Zhang W, Chen Y, Wang X, Shen J, Yang C, Liu X, Deng H. Nicotinamide Mononucleotide agabanya LPS iterwa na LPS na Oxidative Stress binyuze mu kugabanya imvugo ya COX-2 muri Macrophage. Imbere Mol Biosci. 2021 Nyakanga 6.