Oocyte ni intangiriro yubuzima bwabantu, ni selile yamagi idakuze amaherezo ikura mu igi.Nyamara, ubwiza bwa oocyte buragabanuka uko abagore basaza cyangwa bitewe nimpamvu nkumubyibuho ukabije, kandi oocytes yo mu rwego rwo hasi niyo mpamvu nyamukuru itera uburumbuke buke ku bagore bafite umubyibuho ukabije.Nyamara, uburyo bwo kuzamura ireme rya oocytes ku bagore bafite umubyibuho ukabije ni ikibazo ku bahanga.
Vuba aha, ubushakashatsi bwiswe Ubuyobozi bwa nicotinamide mononucleotide butezimbere ubwiza bwa oocyte yimbeba zifite umubyibuho ukabije bwasohotse mu gukwirakwiza selile.Ubushakashatsi bwerekanye ko kuzuza nicotinamide adenine dinucleotide (NAD +) ibanzirizanicotinamide mononucleosideAcide (NMN) irashobora kunoza neza intanga ngore, kuzamura ireme rya oocyte no kugarura uburemere bwumubiri kubyara imbeba z’abagore babyibushye.
Abashakashatsi batoranije imbeba z’abagore zibyumweru 3 nimbeba zibyumweru 11 - kugirango bashireho urugero rwimbeba yumubyibuho ukabije hamwe nimirire yuzuye amavuta kandi byemejwe no gufata ibiro, kwipimisha amaraso glucose, hamwe nikizamini cyo kwihanganira glucose mu kanwa, kwivanga mu mirire 1 Mugihe cibyumweru 2,NMNinyongeramusaruro zatewe muminsi 10 ikurikiranye kugirango hamenyekane imiterere ya genes zifitanye isano niterambere ryintanga ngore hamwe na genes zijyanye no gutwika, ingano yibinure byumubiri wa adipose yo munda, ubwoko bwa ogisijeni ikora ya oocytes, imiterere ya spindlechromosome, Imikorere ya mitochondrial, imikorere ya actin, na ADN yangirika, ugereranije n'ibisubizo by'ibarurishamibare byerekana ：
1. Indyo yuzuye ibinure -yatewe numubyibuho ukabije wimbeba yashizweho neza
Agaciro ka FGB k'ibiryo birimo amavuta menshi (HFD) byahoraga bisumba iby'imirire isanzwe (ND), hiyongereyeho, ibisubizo bya OGTT byerekanaga ko imbeba zifite amavuta menshi (HFD) imbeba zo mu matsinda zidafite glucose.
2. NMN irashobora kunoza metabolike idasanzwe mumbeba za HFD
Inyongera hamweNMNinyongeramusaruro yagabanije ibinure mu mbeba mu itsinda ry’amavuta menshi (HFD), kandi ibisubizo byerekana ko NMN igira ingaruka zo gukingira metabolisme idasanzwe mu mbeba mu itsinda ry’amavuta menshi (HFD).
3. NMN itezimbere intanga ngore mu mbeba za HFD
NMN irashobora kuzamura ubwiza bwintanga mumirire yuzuye ibinure (HFD) muguhindura imvugo ya genes zingenzi zijyanye no gukura kwintanga ngore (Bmp4, Lhx8) no gutwika.
4. NMN igabanya inenge ya oocyte no kwangirika kwa ADN mu mbeba za HFD
NMN irashobora kugabanya inshuro nyinshi inenge ya spindle hamwe na chromosomal idahuye neza iterwa nimirire yuzuye amavuta (HFD), kugabanya ibimenyetso bya γH2A.X, kandi igahindura indyo yuzuye ibinure (HFD) yangiza ADN igabanya imvugo ya Bax.
5. NMN irashobora kuzamura ubwiza bwa oocytes
NMN irashobora gusana imvugo yagabanutse ya antioxydeant SOD1 mumirire yuzuye amavuta (HFD), kunoza ikwirakwizwa rya mitochondriya, kandi irashobora kuzamura ubwiza bwa oocytes ikomeza ubusugire bwa cytoskeleton.
6. NMN irashobora kugarura lipid ibitonyanga mu mbeba za HFD
indyo yuzuye amavuta (HFD) oocytes yo mumatsinda yari hejuru gato ugereranije no mumirire isanzwe (ND) oocytes yitsinda, kandi inyongera ya NMN irashobora kugabanya ubukana bwa fluorescence yibitonyanga bya lipide.
7. NMN igarura uburemere bwumubiri mu rubyaro rwimbeba za HFD
uburemere bw'amavuko bw'urubyaro rw'amavuta menshi (HFD) rwaragabanutse cyane ugereranije n'iribwa risanzwe (ND), kandi kuzuza hamwe na NMN byongeye kugarura uburemere bw'amavuko bw'urubyaro rw'itsinda rya HFD.
Muri ubu bushakashatsi, abashakashatsi berekanye binyuze mu bushakashatsi bw’imbeba ko NMN ifite ubushobozi bwo kuzamura ireme rya oocytes mu mbeba z’abagore bafite umubyibuho ukabije ziterwa n’imirire y’amavuta menshi, maze bagaragaza ko NMN ishobora kugarura imikorere ya mito-iyambere kandi ikagabanya ikwirakwizwa rya ROS muri oocytes y’imbeba zifite umubyibuho ukabije., Kwangiza ADN hamwe nuburyo bwibanze bwo gukwirakwiza lipide.Kubwibyo, ubu bushakashatsi buzatanga ingamba zishoboka zo kuvura mugutezimbere ibibazo byuburumbuke biterwa numubyibuho ukabije kubagore.
Reba ：
1.Wang L, Chen Y, Wei J, n'abandi.Ubuyobozi bwa nicotinamide mononucleotide butezimbere ubwiza bwa oocyte yimbeba zifite umubyibuho ukabije.Akagari ka selile.2022;e13303.doi10.1111cpr.13303
Igihe cyo kohereza: Nzeri-28-2022